Cytopathogenicity markers in the genome of Hungarian cytopathic isolates of bovine viral diarrhoea virus

A. Bálint, Claudia Baule, S. Kecskeméti, I. Kiss, S. Belák

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Since genetic recombination is a major factor in the evolution of the cytopathogenic (cp) bovine viral diarrhoea virus (BVDV) biotypes, in this study the cytopathogenicity markers were investigated in the genomes of two cp BVDV strains recently isolated from mucosal disease (MD) cases in Hungary. In the genome of strain H4956, a Jiv-like insertion was found similar to those described in reference strain NADL and in other BVDV 1, BVDV 2 and border disease virus (BDV) strains. The 133 amino acid Jiv-like sequence is inserted at nucleotide position 4984 (amino acid position 1533), 9 nucleotides upstream of that of strain NADL. The insertion showed 96% amino acid sequence identity with the cellular Jiv protein. In the genome of cp BVDV strain H115/PCR, an ubiquitin-containing duplication was found. The duplicated sequence started at nucleotide position 7978 (amino acid 2531) in the NS4B gene. The duplication contained a complete ubiquitin monomer of 76 amino acids and the complete NS3 gene starting at nucleotide position 5153 (amino acid 1589), which corresponds to the first N-terminal amino acid of NS3. The duplication was located further downstream of the known ubiquitin-containing genomic regions of cp BVDV strains, and it consisted of the shortest inserted nucleotide sequence. The insertions and duplication of strains H4956 and H115/PCR further confirmed that recombinations occurring at positions A and B are the most common mechanisms leading to the development of BVDV cytopathogenicity.

Original languageEnglish
Pages (from-to)125-136
Number of pages12
JournalActa Veterinaria Hungarica
Volume53
Issue number1
DOIs
Publication statusPublished - 2005

Fingerprint

Bovine Viral Diarrhea Viruses
cytopathogenicity
Bovine viral diarrhea virus
Genome
Amino Acids
genome
Nucleotides
Ubiquitin
amino acids
ubiquitin
nucleotides
Genetic Recombination
Border disease virus
Bovine viral diarrhea virus 2
Bovine viral diarrhea virus 1
Polymerase Chain Reaction
Hungary
Virus Diseases
genetic recombination
Genes

Keywords

  • Bovine viral diarrhoea virus
  • Cytopathogenicity
  • Markers
  • NS2-3
  • NS3

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Cytopathogenicity markers in the genome of Hungarian cytopathic isolates of bovine viral diarrhoea virus. / Bálint, A.; Baule, Claudia; Kecskeméti, S.; Kiss, I.; Belák, S.

In: Acta Veterinaria Hungarica, Vol. 53, No. 1, 2005, p. 125-136.

Research output: Contribution to journalArticle

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