Cytometry of raft and caveola membrane microdomains: From flow and imaging techniques to high throughput screening assays

Endre Kiss, Péter Nagy, Andrea Balogh, János Szöllsi, János Matkó

Research output: Contribution to journalReview article

21 Citations (Scopus)


The evolutionarily developed microdomain structure of biological membranes has gained more and more attention in the past decade. The caveolin-free "membrane rafts," the caveolin-expressing rafts (caveolae), as well as other membrane microdomains seem to play an essential role in controlling and coordinating cell-surface molecular recognition, internalization/endocytosis of the bound molecules or pathogenic organisms and in regulation of transmembrane signal transduction processes. Therefore, in many research fields (e.g. neurobiology and immunology), there is an ongoing need to understand the nature of these microdomains and to quantitatively characterize their lipid and protein composition under various physiological and pathological conditions. Flow and image cytometry offer many sophisticated and routine tools to study these questions. In this review, we give an overview of the past efforts to detect and characterize these membrane microdomains by the use of classical cytometric technologies, and finally we will discuss the results and perspectives of a new line of raft cytometry, the "high throughput screening assays of membrane microdomains," based on "lipidomic" and "proteomic" approaches.

Original languageEnglish
Pages (from-to)599-614
Number of pages16
JournalCytometry Part A
Issue number7
Publication statusPublished - Jul 1 2008



  • Flow cytometry of membrane microdomains
  • Imaging rafts
  • Lipidomics and proteomics of rafts
  • Membrane microdomains
  • Rafts and caveolae
  • Structure and composition

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology
  • Cell Biology

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