Cytometry of raft and caveola membrane microdomains: From flow and imaging techniques to high throughput screening assays

Endre Kiss, Péter Nagy, Andrea Balogh, János Szöllsi, J. Matkó

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The evolutionarily developed microdomain structure of biological membranes has gained more and more attention in the past decade. The caveolin-free "membrane rafts," the caveolin-expressing rafts (caveolae), as well as other membrane microdomains seem to play an essential role in controlling and coordinating cell-surface molecular recognition, internalization/endocytosis of the bound molecules or pathogenic organisms and in regulation of transmembrane signal transduction processes. Therefore, in many research fields (e.g. neurobiology and immunology), there is an ongoing need to understand the nature of these microdomains and to quantitatively characterize their lipid and protein composition under various physiological and pathological conditions. Flow and image cytometry offer many sophisticated and routine tools to study these questions. In this review, we give an overview of the past efforts to detect and characterize these membrane microdomains by the use of classical cytometric technologies, and finally we will discuss the results and perspectives of a new line of raft cytometry, the "high throughput screening assays of membrane microdomains," based on "lipidomic" and "proteomic" approaches.

Original languageEnglish
Pages (from-to)599-614
Number of pages16
JournalCytometry Part A
Volume73
Issue number7
DOIs
Publication statusPublished - Jul 2008

Fingerprint

High-Throughput Screening Assays
Membrane Microdomains
Caveolins
Image Cytometry
Membranes
Neurobiology
Endocytosis
Allergy and Immunology
Proteomics
Signal Transduction
Flow Cytometry
Technology
Lipids
Research
Proteins

Keywords

  • Flow cytometry of membrane microdomains
  • Imaging rafts
  • Lipidomics and proteomics of rafts
  • Membrane microdomains
  • Rafts and caveolae
  • Structure and composition

ASJC Scopus subject areas

  • Hematology
  • Cell Biology
  • Pathology and Forensic Medicine
  • Biophysics
  • Endocrinology

Cite this

Cytometry of raft and caveola membrane microdomains : From flow and imaging techniques to high throughput screening assays. / Kiss, Endre; Nagy, Péter; Balogh, Andrea; Szöllsi, János; Matkó, J.

In: Cytometry Part A, Vol. 73, No. 7, 07.2008, p. 599-614.

Research output: Contribution to journalArticle

Kiss, Endre ; Nagy, Péter ; Balogh, Andrea ; Szöllsi, János ; Matkó, J. / Cytometry of raft and caveola membrane microdomains : From flow and imaging techniques to high throughput screening assays. In: Cytometry Part A. 2008 ; Vol. 73, No. 7. pp. 599-614.
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