Cytomegalovirus induced membrane antigens in productively infected cells

I. Boldogh, E. Gönczöl, L. Vaczi

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Adsorbed but not penetrated virus can be removed from the CMV-infected cell membrane by digestion with cystine-activated papain. Membrane antigens appear on 80-90% of the infected cells 14-20 hr after infection as a result of de novo protein synthesis. Antigen synthesis can be blocked with inhibitors of protein synthesis, but not with DNA inhibitors. In the early stage of infection, pooled human convalescent serum reacted well with the membrane antigen, whereas pooled antiserum of rabbits immunized with CMV virion suspension gave a positive reaction with a small proportion of the cells. After the 48th hr, both the human and the rabbit serum pool reacted with the membrane of the infected cells. Adsorption with cells cultured for 24 hr after CMV infection reduced the neutralization titres of the antisera only slightly but the titre reduction was considerable when absorption was performed with cells cultured for more than 48 hr after infection. It is concluded that on the membrane of cells productively infected by CMV at least 2 membrane antigens are present, one coded for by the DNA of the parent virus and another which is the product of the DNA of the virus progeny. The 2 antigens can be differentiated serologically.

Original languageEnglish
Pages (from-to)21-28
Number of pages8
JournalActa Microbiologica Academiae Scientiarum Hungaricae
Volume24
Issue number1
Publication statusPublished - 1977

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Cytomegalovirus
Antigens
Membranes
DNA Viruses
Cell Membrane
Infection
Immune Sera
Cultured Cells
Rabbits
Protein Synthesis Inhibitors
Cystine
Papain
Serum
Virion
Adsorption
Digestion
Suspensions
Viruses
DNA
Proteins

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Cytomegalovirus induced membrane antigens in productively infected cells. / Boldogh, I.; Gönczöl, E.; Vaczi, L.

In: Acta Microbiologica Academiae Scientiarum Hungaricae, Vol. 24, No. 1, 1977, p. 21-28.

Research output: Contribution to journalArticle

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