Cytolethal distending toxin producing Escherichia coli O157: H43 strain T22 represents a novel evolutionary lineage within the O157 serogroup

Domonkos Sváb, Balázs Bálint, Gergely Maróti, I. Tóth

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Enterohemorrhagic Escherichia coli (EHEC) O157:H7/NM strains are significant foodborne pathogens intensively studied, while other sero- and pathotypes of the O157 serogroup only began to receive more attention. Here we report the first genome sequence of a cytolethal distending toxin (CDT-V) producing E. coli O157:H43 strain (T22) isolated from cattle. The genome consists of a 4.9 Mb chromosome assembled into three contigs and one plasmid of 82.4 kb. Comparative genomic investigations conducted with the core genomes of representative E. coli strains in GenBank (n = 62) confirmed the separation of T22 from the EHEC and enteropathogenic (EPEC) O157 lineages. Gene content based pangenome analysis revealed as many as 261 T22-specific coding sequences without orthologs in EDL933 EHEC O157 prototypic and two phylogenetically related commensal E. coli strains. The genome sequence revealed 10 prophage-like regions which harbor several virulence-associated genes including cdt and heat-labile enterotoxin (LT-II) encoding operons. Our results indicate that the evolutionary path of T22 is largely independent from that of EHEC and EPEC O157:H7/NM strains. Thus, the CDT-producing T22 E. coli O157:H43 strain represents a unique lineage of E. coli O157.

Original languageEnglish
Pages (from-to)110-117
Number of pages8
JournalInfection, Genetics and Evolution
Volume46
DOIs
Publication statusPublished - Dec 1 2016

Fingerprint

Escherichia coli O157
toxin
enterohemorrhagic Escherichia coli
serotypes
toxins
genome
Enterohemorrhagic Escherichia coli
Genome
pathotype
commensal
gene
virulence
plasmid
Escherichia coli
cattle
chromosome
genomics
harbor
pathogen
Prophages

Keywords

  • CDT
  • Comparative genomics
  • Escherichia
  • LT-II
  • O157:H43
  • Phylogeny

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Genetics
  • Microbiology (medical)
  • Infectious Diseases

Cite this

Cytolethal distending toxin producing Escherichia coli O157 : H43 strain T22 represents a novel evolutionary lineage within the O157 serogroup. / Sváb, Domonkos; Bálint, Balázs; Maróti, Gergely; Tóth, I.

In: Infection, Genetics and Evolution, Vol. 46, 01.12.2016, p. 110-117.

Research output: Contribution to journalArticle

@article{fb6d099d95ff43848be400d0b71b5d0f,
title = "Cytolethal distending toxin producing Escherichia coli O157: H43 strain T22 represents a novel evolutionary lineage within the O157 serogroup",
abstract = "Enterohemorrhagic Escherichia coli (EHEC) O157:H7/NM strains are significant foodborne pathogens intensively studied, while other sero- and pathotypes of the O157 serogroup only began to receive more attention. Here we report the first genome sequence of a cytolethal distending toxin (CDT-V) producing E. coli O157:H43 strain (T22) isolated from cattle. The genome consists of a 4.9 Mb chromosome assembled into three contigs and one plasmid of 82.4 kb. Comparative genomic investigations conducted with the core genomes of representative E. coli strains in GenBank (n = 62) confirmed the separation of T22 from the EHEC and enteropathogenic (EPEC) O157 lineages. Gene content based pangenome analysis revealed as many as 261 T22-specific coding sequences without orthologs in EDL933 EHEC O157 prototypic and two phylogenetically related commensal E. coli strains. The genome sequence revealed 10 prophage-like regions which harbor several virulence-associated genes including cdt and heat-labile enterotoxin (LT-II) encoding operons. Our results indicate that the evolutionary path of T22 is largely independent from that of EHEC and EPEC O157:H7/NM strains. Thus, the CDT-producing T22 E. coli O157:H43 strain represents a unique lineage of E. coli O157.",
keywords = "CDT, Comparative genomics, Escherichia, LT-II, O157:H43, Phylogeny",
author = "Domonkos Sv{\'a}b and Bal{\'a}zs B{\'a}lint and Gergely Mar{\'o}ti and I. T{\'o}th",
year = "2016",
month = "12",
day = "1",
doi = "10.1016/j.meegid.2016.11.003",
language = "English",
volume = "46",
pages = "110--117",
journal = "Infection, Genetics and Evolution",
issn = "1567-1348",
publisher = "Elsevier",

}

TY - JOUR

T1 - Cytolethal distending toxin producing Escherichia coli O157

T2 - H43 strain T22 represents a novel evolutionary lineage within the O157 serogroup

AU - Sváb, Domonkos

AU - Bálint, Balázs

AU - Maróti, Gergely

AU - Tóth, I.

PY - 2016/12/1

Y1 - 2016/12/1

N2 - Enterohemorrhagic Escherichia coli (EHEC) O157:H7/NM strains are significant foodborne pathogens intensively studied, while other sero- and pathotypes of the O157 serogroup only began to receive more attention. Here we report the first genome sequence of a cytolethal distending toxin (CDT-V) producing E. coli O157:H43 strain (T22) isolated from cattle. The genome consists of a 4.9 Mb chromosome assembled into three contigs and one plasmid of 82.4 kb. Comparative genomic investigations conducted with the core genomes of representative E. coli strains in GenBank (n = 62) confirmed the separation of T22 from the EHEC and enteropathogenic (EPEC) O157 lineages. Gene content based pangenome analysis revealed as many as 261 T22-specific coding sequences without orthologs in EDL933 EHEC O157 prototypic and two phylogenetically related commensal E. coli strains. The genome sequence revealed 10 prophage-like regions which harbor several virulence-associated genes including cdt and heat-labile enterotoxin (LT-II) encoding operons. Our results indicate that the evolutionary path of T22 is largely independent from that of EHEC and EPEC O157:H7/NM strains. Thus, the CDT-producing T22 E. coli O157:H43 strain represents a unique lineage of E. coli O157.

AB - Enterohemorrhagic Escherichia coli (EHEC) O157:H7/NM strains are significant foodborne pathogens intensively studied, while other sero- and pathotypes of the O157 serogroup only began to receive more attention. Here we report the first genome sequence of a cytolethal distending toxin (CDT-V) producing E. coli O157:H43 strain (T22) isolated from cattle. The genome consists of a 4.9 Mb chromosome assembled into three contigs and one plasmid of 82.4 kb. Comparative genomic investigations conducted with the core genomes of representative E. coli strains in GenBank (n = 62) confirmed the separation of T22 from the EHEC and enteropathogenic (EPEC) O157 lineages. Gene content based pangenome analysis revealed as many as 261 T22-specific coding sequences without orthologs in EDL933 EHEC O157 prototypic and two phylogenetically related commensal E. coli strains. The genome sequence revealed 10 prophage-like regions which harbor several virulence-associated genes including cdt and heat-labile enterotoxin (LT-II) encoding operons. Our results indicate that the evolutionary path of T22 is largely independent from that of EHEC and EPEC O157:H7/NM strains. Thus, the CDT-producing T22 E. coli O157:H43 strain represents a unique lineage of E. coli O157.

KW - CDT

KW - Comparative genomics

KW - Escherichia

KW - LT-II

KW - O157:H43

KW - Phylogeny

UR - http://www.scopus.com/inward/record.url?scp=84994831324&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84994831324&partnerID=8YFLogxK

U2 - 10.1016/j.meegid.2016.11.003

DO - 10.1016/j.meegid.2016.11.003

M3 - Article

C2 - 27826029

AN - SCOPUS:84994831324

VL - 46

SP - 110

EP - 117

JO - Infection, Genetics and Evolution

JF - Infection, Genetics and Evolution

SN - 1567-1348

ER -