Cytokines and adhesion molecules in duodenal mucosa of children with delayed-type food allergy

Gabor Veres, Mia Westerholm-Ormio, Jorma Kokkonen, Andras Arato, Erkki Savilahti

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Objectives: The aim was to investigate the expression of cytokines, adhesion molecules, and activation and proliferation markers in duodenal biopsies from children with delayed-type food allergy (FA). Methods: Seven children with untreated FA (uFA), seven children with treated FA (tFA) to cow milk and/or cereals, and five normal controls furnished duodenal biopsy specimens. Additionally, five pediatric patients with celiac disease were included, serving exclusively as positive controls for in situ hybridization. Interferon-γ (IFN-γ), interleukin-4 (IL-4), adhesion molecules, and activation markers were detected by immunohistochemistry, and expression of IFN-γ and IL-4 messenger RNA was revealed by in situ hybridization. Results: uFA patients had a higher density of IFN-γ positive cells in the lamina propria than did tFA patients and controls (P = 0.053 and P = 0.018). Moreover, the uFA patients exhibited a higher proportion of crypt cells in mitosis than did tFA patients (P = 0.026), and stronger staining of HLA-DR in the crypts and increased density of γδ-T cell receptor-positive intraepithelial lymphocytes than did controls (P = 0.048 and P = 0.010). The densities of α4β7 positive cells in the lamina propria tended to be higher in controls than in uFA or tFA patients (P = 0.106, P = 0.073). Expression of IL-4 mRNA was significantly higher in celiac patients than in the other study groups (uFA P = 0.006, tFA P = 0.010; controls P = 0.029), and celiac patients showed higher expression of IFN-γ mRNA than did tFA patients or controls (P = 0.017 and P = 0.016). Conclusions: As expected, Thl dominance was present in the lamina propria of children with delayed-type FA. It may cause activation of epithelial cells and increase their turnover.

Original languageEnglish
Pages (from-to)27-34
Number of pages8
JournalJournal of pediatric gastroenterology and nutrition
Volume37
Issue number1
DOIs
Publication statusPublished - Jul 2003

Keywords

  • Adhesion molecules
  • Cytokines
  • Delayed-type food allergy
  • T cell subset

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health
  • Gastroenterology

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