Cytogenetic and molecular localization of tipE: A gene affecting sodium channels in Drosophila melanogaster

G. Feng, P. Deák, D. P. Kasbekar, D. W. Gil, L. M. Hall

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Voltage-sensitive sodium channels play a key role in nerve cells where they are responsible for the increase in sodium permeability during the rising phase of action potentials. In Drosophila melanogaster a subset of temperature-sensitive paralytic mutations affect sodium channel function. One such mutation is temperature-induced paralysis locus E (tipE), which has been shown by electrophysiology and ligand binding studies to reduce sodium channel numbers. Three new γ-ray-induced tipE alleles associated with either visible deletions in 64AB or a translocation breakpoint within 64B2 provide landmarks for positional cloning of tipE. Beginning with the flanking cloned gene Ras2, a 140-kb walk across the translocation breakpoint was completed. Germline transformation using a 42-kb cosmid clone and successively smaller subclones localized the tipE gene within a 7.4-kb genomic DNA segment. Although this chromosome region is rich in transcripts, only three overlapping mRNAs (5.4, 4.4, and 1.7 kb) lie completely within the smallest rescuing construct. The small sizes of the rescuing construct and transcripts suggest that tipE does not encode a standard sodium channel α-subunit with four homologous repeats. Sequencing these transcripts will elucidate the role of the tipE gene product in sodium channel functional regulation.

Original languageEnglish
Pages (from-to)1679-1688
Number of pages10
JournalGenetics
Volume139
Issue number4
Publication statusPublished - 1995

Fingerprint

Sodium Channels
Drosophila melanogaster
Cytogenetics
Paralysis
Temperature
Genes
Cosmids
Mutation
Electrophysiology
Action Potentials
Organism Cloning
Permeability
Clone Cells
Chromosomes
Sodium
Alleles
Ligands
Neurons
Messenger RNA
DNA

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Cite this

Feng, G., Deák, P., Kasbekar, D. P., Gil, D. W., & Hall, L. M. (1995). Cytogenetic and molecular localization of tipE: A gene affecting sodium channels in Drosophila melanogaster. Genetics, 139(4), 1679-1688.

Cytogenetic and molecular localization of tipE : A gene affecting sodium channels in Drosophila melanogaster. / Feng, G.; Deák, P.; Kasbekar, D. P.; Gil, D. W.; Hall, L. M.

In: Genetics, Vol. 139, No. 4, 1995, p. 1679-1688.

Research output: Contribution to journalArticle

Feng, G, Deák, P, Kasbekar, DP, Gil, DW & Hall, LM 1995, 'Cytogenetic and molecular localization of tipE: A gene affecting sodium channels in Drosophila melanogaster', Genetics, vol. 139, no. 4, pp. 1679-1688.
Feng, G. ; Deák, P. ; Kasbekar, D. P. ; Gil, D. W. ; Hall, L. M. / Cytogenetic and molecular localization of tipE : A gene affecting sodium channels in Drosophila melanogaster. In: Genetics. 1995 ; Vol. 139, No. 4. pp. 1679-1688.
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