Cytochemical correlates of the sleep-wake interface: Concerted expression of brain-derived nitric oxide synthase (bNOS) and the nicotinic acetylcholine receptor (nAChR) in a columnoid organization of the primate prefrontal cortex

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Abstract

Nitric oxide (NO) was recently proposed to be involved in the sleep-wake cycle and cortical spreading depression. As a structural correlate of these functions, we found that bNOS IR was expressed by three cell types in the prefrontal cortex, viz. bipolar, multipolar, and stellate cells. Dendrites of bipolar cells established bundles resulting in a columnoid organization; in addition, the monoclonal antibody mAb 35 which labels subunits α1, α3 and α5 of nAChR, also visualized apical axons proceeding alongside the columnoids. In contrast, α-bungarotoxin which labels the α7-subunit of nAChR, visualized only perikarya of interneurons from where the apical axons arose. In the prefrontal cortex of monkeys which were anesthetized for 6-24 hours, only traces of the columnoid organization were found, while perikaryal bNOS and nAChR were invariably expressed. It is suggested that interactions between NO and presynaptically released ACh might be involved in cortical functions such as the sleep/wake cycle.

Original languageEnglish
Pages (from-to)217-221
Number of pages5
JournalAnnals of Anatomy
Volume186
Issue number3
DOIs
Publication statusPublished - Jun 2004

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Keywords

  • Acetylcholine receptor
  • Macaca fascicularis
  • Nitric oxide synthase
  • Prefrontal cortex
  • Sleep/wake interface

ASJC Scopus subject areas

  • Anatomy
  • Developmental Biology

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