Cylindrospermopsin and microcystin-LR alter the growth, development and peroxidase enzyme activity of white mustard (Sinapis alba L.) seedlings, a comparative analysis

Márta M.-Hamvas, C. Máthé, G. Vasas, Katalin Jámbrik, Mária Papp, D. Beyer, I. Mészáros, G. Borbély

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

This work focuses on the comparative analysis of the effects of two cyanobacterial toxins of different chemical structure cylindrospermopsin (CYN) and microcystin-LR (MC-LR) on the white mustard (Sinapis alba L.) seedlings. Both cyanotoxins reduced significantly the fresh mass and the length of cotyledons, hypocotyls and main roots of seedlings in a concentration dependent manner. For various mustard organs the 50% inhibitory concentration values (IC50) of growth were between 3-5 μg ml-1 for MC-LR and between 5-10 μg ml-1 for CYN, respectively. Cyanotoxins altered the development of cotyledons, the accumulation of photosynthetically active pigments and anthocyanins. Low MC-LR concentrations (0.01 and 0.1 μg ml -1) stimulated anthocyanin formation in the cotyledons but higher than 1 μg ml-1 MC-LR concentrations strongly inhibited it. The CYN treated chlorotic cotyledons were violet coloured in consequence of high level of anthocyanins, while MC-LR induced chlorosis was accompanied by the appearance of necrotic patches. Necrosis and increases of peroxidase enzyme activity (POD) are general stress responses but these alterations were characteristic only for MC-LR treated mustard plants. These findings provide experimental evidences of developmental alterations induced by protein synthesis and protein phosphatase inhibitory cyanotoxins (CYN and MC-LR) in a model dicotyledonous plant.

Original languageEnglish
Pages (from-to)35-48
Number of pages14
JournalActa Biologica Hungarica
Volume61
Issue numberSUPPL. 1
DOIs
Publication statusPublished - Jan 1 2010

Fingerprint

Sinapis
Enzyme activity
Seedlings
Growth and Development
growth and development
Peroxidase
enzyme activity
seedling
Cotyledon
protein
Enzymes
Anthocyanins
phosphatase
toxin
pigment
Mustard Plant
Inhibitory Concentration 50
Hypochromic Anemia
Hypocotyl
Phosphoprotein Phosphatases

Keywords

  • anthocyanin
  • Cylindrospermopsin
  • Microcystin-LR
  • POD
  • Sinapis alba L.

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Environmental Science(all)
  • Neurology

Cite this

Cylindrospermopsin and microcystin-LR alter the growth, development and peroxidase enzyme activity of white mustard (Sinapis alba L.) seedlings, a comparative analysis. / M.-Hamvas, Márta; Máthé, C.; Vasas, G.; Jámbrik, Katalin; Papp, Mária; Beyer, D.; Mészáros, I.; Borbély, G.

In: Acta Biologica Hungarica, Vol. 61, No. SUPPL. 1, 01.01.2010, p. 35-48.

Research output: Contribution to journalArticle

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AU - Vasas, G.

AU - Jámbrik, Katalin

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AU - Mészáros, I.

AU - Borbély, G.

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