Cyclodextrins Are Likely to Induce Cyclodextrin Glycosyltransferase Production in Bacillus macerans

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Abstract

Cyclodextrin glycosyltransferase (CGTase) activity was monitored in Bacillus macerans culture fluids up to 56 h incubation time using glucose (G1), maltose (G2), maltotriose (G3), maltoheptaose (G7), α-, β-, γ-cyclodextrins (CDs) and soluble starch as carbon sources. Highest maximum specific growth rates (μmax) were observed with glucose, γ-CD and soluble starch (μmax values were 0.86, 0.74 and 0.69/h, respectively) while the maximum viable cell numbers were always within the range of 2.3-7.1 × 1011 CFU/mL independently of the carbon source used. Highest CGTase production was found in the presence of soluble starch and G7 (55.0 and 35.4 nkat/mL, respectively), these saccharides being easily transformed to CDs by CGTase. Moreover, when culture media were supplemented with cyclic malto-oligosaccharides the CGTase activities were about twice higher (19.6-20.6 nkat/mL) than those obtained with the linear G2 and G3 saccharides (8.9 and 11.3 nkat/mL, respectively) which give rise only to negligible quantities of CDs. CDs, which are the major end products of the action of CGTase, are regarded thus as the likely physiological inducers of the enzyme.

Original languageEnglish
Pages (from-to)71-74
Number of pages4
JournalFolia Microbiologica
Volume43
Issue number1
Publication statusPublished - 1998

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Cyclodextrins
Bacillus
Starch
Carbon
Glucose
Maltose
Oligosaccharides
Culture Media
Cell Count
cyclomaltodextrin glucanotransferase
Enzymes
Growth

ASJC Scopus subject areas

  • Microbiology

Cite this

Cyclodextrins Are Likely to Induce Cyclodextrin Glycosyltransferase Production in Bacillus macerans. / Pócsi, I.; Nógrády, N.; Lipták, A.; Szentirmai, A.

In: Folia Microbiologica, Vol. 43, No. 1, 1998, p. 71-74.

Research output: Contribution to journalArticle

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