Cyclic nucleotides, the photosynthetic apparatus and response to a UV-B stress in the cyanobacterium Synechocystis sp. PCC 6803

Jean Charles Cadoret, Bernard Rousseau, Irène Perewoska, Cosmin Sicora, Otilia Cheregi, I. Vass, Jean Houmard

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Cyclic nucleotides cAMP and cGMP are ubiquitous signaling molecules that mediate many adaptative responses in eukaryotic cells. Cyanobacteria present the peculiarity among the prokaryotes of having the two types of cyclic nucleotide. Cellular homeostasis requires both cyclases (adenylyl/guanylyl, for their synthesis) and phosphodiesterases (for their degradation). Fully segregated null mutants have been obtained for the two genes, sll1624 and slr2100, which encode putative cNMP phosphodiesterases. We present physiological evidence that the Synechocystis PCC 6803 open reading frame slr2100 could be a cGMP phosphodiesterase. In addition, we show that Slr2100, but not Sll1624, is required for the adaptation of the cells to a UV-B stress. UV-B radiation has deleterious effects for photosynthetic organisms, in particular on the photosystem II, through damaging the protein structure of the reaction center. Using biophysical and biochemical approaches, it was found that Slr2100 is involved in the signal transduction events which permit the repair of the UV-B-damaged photosystem II. This was confirmed by quantitative reverse transcriptase-PCR analyses. Altogether, the data point to an important role for cGMP in signal transduction and photoacclimation processes during a UV-B stress.

Original languageEnglish
Pages (from-to)33935-33944
Number of pages10
JournalJournal of Biological Chemistry
Volume280
Issue number40
DOIs
Publication statusPublished - Oct 7 2005

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Synechocystis
Cyclic Nucleotides
Phosphoric Diester Hydrolases
Cyanobacteria
Signal transduction
Photosystem II Protein Complex
Signal Transduction
Guanylate Cyclase
RNA-Directed DNA Polymerase
Eukaryotic Cells
Reverse Transcriptase Polymerase Chain Reaction
Adenylyl Cyclases
Open Reading Frames
Homeostasis
Repair
Genes
Radiation
Degradation
Molecules
Proteins

ASJC Scopus subject areas

  • Biochemistry

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Cyclic nucleotides, the photosynthetic apparatus and response to a UV-B stress in the cyanobacterium Synechocystis sp. PCC 6803. / Cadoret, Jean Charles; Rousseau, Bernard; Perewoska, Irène; Sicora, Cosmin; Cheregi, Otilia; Vass, I.; Houmard, Jean.

In: Journal of Biological Chemistry, Vol. 280, No. 40, 07.10.2005, p. 33935-33944.

Research output: Contribution to journalArticle

Cadoret, Jean Charles ; Rousseau, Bernard ; Perewoska, Irène ; Sicora, Cosmin ; Cheregi, Otilia ; Vass, I. ; Houmard, Jean. / Cyclic nucleotides, the photosynthetic apparatus and response to a UV-B stress in the cyanobacterium Synechocystis sp. PCC 6803. In: Journal of Biological Chemistry. 2005 ; Vol. 280, No. 40. pp. 33935-33944.
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