Cyclic GMP excretion blocked by isatin administration under conditions of fluid overload

Alexei Medvedev, Agnes Adamik, Vivette Glover, G. Telegdy

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Isatin is a potent inhibitor of atrial natriuretic peptide (ANP) receptors and ANP-induced generation of cGMP in vitro. This study was designed to determine whether it had a similar effect in vivo, using a model of fluid overload known to induce ANP. We confirmed that this model increased urinary output of cGMP 3 hr after volume loading, and showed that this effect was blocked by i.p. injection of isatin (50 mg/kg). Isatin had no effect on urine volume or sodium output. However, isatin did have an effect on plasma protein concentration, both compared with control values, compatible with shifting fluid to the vascular compartment, and after volume overload, in which it normalised such a shift. Isatin thus affected both the generation of cGMP and fluid balance in vivo.

Original languageEnglish
Pages (from-to)225-227
Number of pages3
JournalBiochemical Pharmacology
Volume62
Issue number2
DOIs
Publication statusPublished - Jul 15 2001

Fingerprint

Isatin
Cyclic GMP
Fluids
Atrial Natriuretic Factor
Atrial Natriuretic Factor Receptors
Water-Electrolyte Balance
Blood Vessels
Blood Proteins
Sodium
Urine
Injections

Keywords

  • ANP
  • cGMP
  • Isatin
  • Plasma
  • Rat
  • Water balance

ASJC Scopus subject areas

  • Pharmacology

Cite this

Cyclic GMP excretion blocked by isatin administration under conditions of fluid overload. / Medvedev, Alexei; Adamik, Agnes; Glover, Vivette; Telegdy, G.

In: Biochemical Pharmacology, Vol. 62, No. 2, 15.07.2001, p. 225-227.

Research output: Contribution to journalArticle

Medvedev, Alexei ; Adamik, Agnes ; Glover, Vivette ; Telegdy, G. / Cyclic GMP excretion blocked by isatin administration under conditions of fluid overload. In: Biochemical Pharmacology. 2001 ; Vol. 62, No. 2. pp. 225-227.
@article{e7a5ae71316c46a2969a1364a03faed3,
title = "Cyclic GMP excretion blocked by isatin administration under conditions of fluid overload",
abstract = "Isatin is a potent inhibitor of atrial natriuretic peptide (ANP) receptors and ANP-induced generation of cGMP in vitro. This study was designed to determine whether it had a similar effect in vivo, using a model of fluid overload known to induce ANP. We confirmed that this model increased urinary output of cGMP 3 hr after volume loading, and showed that this effect was blocked by i.p. injection of isatin (50 mg/kg). Isatin had no effect on urine volume or sodium output. However, isatin did have an effect on plasma protein concentration, both compared with control values, compatible with shifting fluid to the vascular compartment, and after volume overload, in which it normalised such a shift. Isatin thus affected both the generation of cGMP and fluid balance in vivo.",
keywords = "ANP, cGMP, Isatin, Plasma, Rat, Water balance",
author = "Alexei Medvedev and Agnes Adamik and Vivette Glover and G. Telegdy",
year = "2001",
month = "7",
day = "15",
doi = "10.1016/S0006-2952(01)00662-1",
language = "English",
volume = "62",
pages = "225--227",
journal = "Biochemical Pharmacology",
issn = "0006-2952",
publisher = "Elsevier Inc.",
number = "2",

}

TY - JOUR

T1 - Cyclic GMP excretion blocked by isatin administration under conditions of fluid overload

AU - Medvedev, Alexei

AU - Adamik, Agnes

AU - Glover, Vivette

AU - Telegdy, G.

PY - 2001/7/15

Y1 - 2001/7/15

N2 - Isatin is a potent inhibitor of atrial natriuretic peptide (ANP) receptors and ANP-induced generation of cGMP in vitro. This study was designed to determine whether it had a similar effect in vivo, using a model of fluid overload known to induce ANP. We confirmed that this model increased urinary output of cGMP 3 hr after volume loading, and showed that this effect was blocked by i.p. injection of isatin (50 mg/kg). Isatin had no effect on urine volume or sodium output. However, isatin did have an effect on plasma protein concentration, both compared with control values, compatible with shifting fluid to the vascular compartment, and after volume overload, in which it normalised such a shift. Isatin thus affected both the generation of cGMP and fluid balance in vivo.

AB - Isatin is a potent inhibitor of atrial natriuretic peptide (ANP) receptors and ANP-induced generation of cGMP in vitro. This study was designed to determine whether it had a similar effect in vivo, using a model of fluid overload known to induce ANP. We confirmed that this model increased urinary output of cGMP 3 hr after volume loading, and showed that this effect was blocked by i.p. injection of isatin (50 mg/kg). Isatin had no effect on urine volume or sodium output. However, isatin did have an effect on plasma protein concentration, both compared with control values, compatible with shifting fluid to the vascular compartment, and after volume overload, in which it normalised such a shift. Isatin thus affected both the generation of cGMP and fluid balance in vivo.

KW - ANP

KW - cGMP

KW - Isatin

KW - Plasma

KW - Rat

KW - Water balance

UR - http://www.scopus.com/inward/record.url?scp=0035879928&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035879928&partnerID=8YFLogxK

U2 - 10.1016/S0006-2952(01)00662-1

DO - 10.1016/S0006-2952(01)00662-1

M3 - Article

C2 - 11389882

AN - SCOPUS:0035879928

VL - 62

SP - 225

EP - 227

JO - Biochemical Pharmacology

JF - Biochemical Pharmacology

SN - 0006-2952

IS - 2

ER -