Cyclic electron flow around PSI monitored by afterglow luminescence in leaves of maize inbred lines (Zea mays L.): Correlation with chilling tolerance

Jean Marc Ducruet, Miruna Roman, Michel Havaux, Tibor Janda, André Gallais

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Maize (Zea mays L.) inbred lines of contrasting chilling sensitivity (three tolerant, three sensitive lines) were acclimated to 280 μmol photons m -2 s-1 white light at a 17°C sub-optimal temperature. They showed no symptoms of photoinhibition, despite slight changes in photosystem II (PSII) fluorescence and thermoluminescence properties in two tolerant lines. A luminescence "afterglow" emission [Bertsch and Azzi (1965) Biochim Biophys Acta 94:15-26], inducible by a far-red (FR) illumination of unfrozen leaf discs, was detected either as a bounce in decay kinetics at constant temperatures or as a sharp thermoluminescence afterglow band at about 45°C, in dark-adapted leaves. This band reflects the induction by warming of an electron pathway from stromal reductants to plastoquinones and to the Q B secondary acceptor of PSII, resulting in a luminescence-emitting charge recombination in the fraction of centres that were initially in the S2/3QB non-luminescent state. A 5-h exposure of plants to growth chamber light shifted this luminescence emission towards shorter times and lower temperatures for several hours in the three chilling-tolerant lines. This downshift was not observed, or only transiently, in the three sensitive lines. In darkness, the downshifted afterglow band relaxed within hours to resume its dark-adapted location, similar for all maize lines. A faster dark re-reduction of P700+ oxidized by FR light (monitored by 820-nm absorbance) and an increase of photochemical energy storage under FR excitation (determined by photoacoustic spectroscopy) confirmed that a cyclic pathway induced by white actinic light remained activated for several hours in the tolerant maize lines.

Original languageEnglish
Pages (from-to)567-579
Number of pages13
JournalPlanta
Volume221
Issue number4
DOIs
Publication statusPublished - Jun 2005

Keywords

  • Chlororespiration
  • Ferredoxine-plastoquinone-reductase
  • Photoinhibition
  • Photosystem I-cyclic electron transfer
  • Photosystem II
  • Thermoluminescence

ASJC Scopus subject areas

  • Genetics
  • Plant Science

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