Cyclic AMP influences protein synthesis in larval brains of Drosophila melanogaster.

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Abstract

The protein synthesis in dissected whole larval brains of Drosophila melanogaster has been monitored by [35S] methionine incorporation, as revealed by two-dimensional gel-electrophoresis and fluorography. In wild type brains, drugs known to increase cAMP level increased the labelling of at least two proteins in the Mr range 30 to 120 kD and pI range 4.8 to 6.2. One of these proteins, Mr = 78 kD and pI = 5.9, was also enhanced in the dunceM11 memory-mutant, which has an elevated cAMP level, whereas it was hardly affected in the rutabaga memory-mutant, which has a subnormal cAMP level. It is suggested that cAMP-induced alterations in protein composition and/or turnover of nerve cells may contribute to the development of memory deficit in the dunce strains.

Original languageEnglish
Pages (from-to)61-68
Number of pages8
JournalActa Biochimica et Biophysica Hungarica
Volume24
Issue number1-2
Publication statusPublished - 1989

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Drosophila melanogaster
Cyclic AMP
Brain
Data storage equipment
Photofluorography
Proteins
Brassica napus
Memory Disorders
Electrophoresis, Gel, Two-Dimensional
Electrophoresis
Methionine
Labeling
Neurons
Gels
Chemical analysis
Pharmaceutical Preparations

ASJC Scopus subject areas

  • Biochemistry

Cite this

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abstract = "The protein synthesis in dissected whole larval brains of Drosophila melanogaster has been monitored by [35S] methionine incorporation, as revealed by two-dimensional gel-electrophoresis and fluorography. In wild type brains, drugs known to increase cAMP level increased the labelling of at least two proteins in the Mr range 30 to 120 kD and pI range 4.8 to 6.2. One of these proteins, Mr = 78 kD and pI = 5.9, was also enhanced in the dunceM11 memory-mutant, which has an elevated cAMP level, whereas it was hardly affected in the rutabaga memory-mutant, which has a subnormal cAMP level. It is suggested that cAMP-induced alterations in protein composition and/or turnover of nerve cells may contribute to the development of memory deficit in the dunce strains.",
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