Cyclic AMP-induced phosphorylation of 27.5-kda protein(s) in larval brains of normal and memory-mutant drosophila melanogaster

Piroska Dévay, Peter Friedrich

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Whole brains dissected from third-instar larvae of Drosophila melanogaster increase their cAMP content when incubated with octopamine, dopamine and serotonin, especially in the presence of theophylline, a phosphodiesterase inhibitor. The most pronounced rise in cAMP was produced by forskolin. When brains prelabelled with [32P]orthophosphate were subjected to these treatments, the increased labelling of 27.5-kDa protein(s) of pi 6.3-6.6 (substrate A) was observed in all cases, as revealed by two-dimensional gel electrophoresis and autoradiography. Labelling of substrate A was enhanced without any drug addition in brains of the dunceM11 mutant strain, which has abnormally high basal cAMP levels. The labelling of substrate A was also promoted by the potassium channel blocker 4-aminopyridine. The data suggest that in Drosophila larval brain the 27.5-kDa protein is a target of cAMP-triggered phosphorylation and may be related to potassium channel(s).

Original languageEnglish
Pages (from-to)275-284
Number of pages10
JournalJournal of Neurogenetics
Volume4
Issue number1
DOIs
Publication statusPublished - Jan 1 1987

Keywords

  • CAMP-dependent protein kinase
  • Drosophila
  • Larval brain
  • Memory mutant dunce
  • Potassium channel
  • Protein phosphorylation

ASJC Scopus subject areas

  • Genetics
  • Cellular and Molecular Neuroscience

Fingerprint Dive into the research topics of 'Cyclic AMP-induced phosphorylation of 27.5-kda protein(s) in larval brains of normal and memory-mutant drosophila melanogaster'. Together they form a unique fingerprint.

  • Cite this