Cyclic AMP-induced phosphorylation of 27.5-kda protein(s) in larval brains of normal and memory-mutant drosophila melanogaster

Piroska Dévay, P. Friedrich

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Whole brains dissected from third-instar larvae of Drosophila melanogaster increase their cAMP content when incubated with octopamine, dopamine and serotonin, especially in the presence of theophylline, a phosphodiesterase inhibitor. The most pronounced rise in cAMP was produced by forskolin. When brains prelabelled with [32P]orthophosphate were subjected to these treatments, the increased labelling of 27.5-kDa protein(s) of pi 6.3-6.6 (substrate A) was observed in all cases, as revealed by two-dimensional gel electrophoresis and autoradiography. Labelling of substrate A was enhanced without any drug addition in brains of the dunceM11 mutant strain, which has abnormally high basal cAMP levels. The labelling of substrate A was also promoted by the potassium channel blocker 4-aminopyridine. The data suggest that in Drosophila larval brain the 27.5-kDa protein is a target of cAMP-triggered phosphorylation and may be related to potassium channel(s).

Original languageEnglish
Pages (from-to)275-284
Number of pages10
JournalJournal of Neurogenetics
Volume4
Issue number1
DOIs
Publication statusPublished - 1987

Fingerprint

Drosophila melanogaster
Cyclic AMP
Phosphorylation
Brain
Proteins
Potassium Channel Blockers
Octopamine
4-Aminopyridine
Phosphodiesterase Inhibitors
Potassium Channels
Electrophoresis, Gel, Two-Dimensional
Colforsin
Theophylline
Autoradiography
Drosophila
Larva
Dopamine
Serotonin
Phosphates
Pharmaceutical Preparations

Keywords

  • CAMP-dependent protein kinase
  • Drosophila
  • Larval brain
  • Memory mutant dunce
  • Potassium channel
  • Protein phosphorylation

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Genetics
  • Developmental Biology
  • Neuroscience(all)

Cite this

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abstract = "Whole brains dissected from third-instar larvae of Drosophila melanogaster increase their cAMP content when incubated with octopamine, dopamine and serotonin, especially in the presence of theophylline, a phosphodiesterase inhibitor. The most pronounced rise in cAMP was produced by forskolin. When brains prelabelled with [32P]orthophosphate were subjected to these treatments, the increased labelling of 27.5-kDa protein(s) of pi 6.3-6.6 (substrate A) was observed in all cases, as revealed by two-dimensional gel electrophoresis and autoradiography. Labelling of substrate A was enhanced without any drug addition in brains of the dunceM11 mutant strain, which has abnormally high basal cAMP levels. The labelling of substrate A was also promoted by the potassium channel blocker 4-aminopyridine. The data suggest that in Drosophila larval brain the 27.5-kDa protein is a target of cAMP-triggered phosphorylation and may be related to potassium channel(s).",
keywords = "CAMP-dependent protein kinase, Drosophila, Larval brain, Memory mutant dunce, Potassium channel, Protein phosphorylation",
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T1 - Cyclic AMP-induced phosphorylation of 27.5-kda protein(s) in larval brains of normal and memory-mutant drosophila melanogaster

AU - Dévay, Piroska

AU - Friedrich, P.

PY - 1987

Y1 - 1987

N2 - Whole brains dissected from third-instar larvae of Drosophila melanogaster increase their cAMP content when incubated with octopamine, dopamine and serotonin, especially in the presence of theophylline, a phosphodiesterase inhibitor. The most pronounced rise in cAMP was produced by forskolin. When brains prelabelled with [32P]orthophosphate were subjected to these treatments, the increased labelling of 27.5-kDa protein(s) of pi 6.3-6.6 (substrate A) was observed in all cases, as revealed by two-dimensional gel electrophoresis and autoradiography. Labelling of substrate A was enhanced without any drug addition in brains of the dunceM11 mutant strain, which has abnormally high basal cAMP levels. The labelling of substrate A was also promoted by the potassium channel blocker 4-aminopyridine. The data suggest that in Drosophila larval brain the 27.5-kDa protein is a target of cAMP-triggered phosphorylation and may be related to potassium channel(s).

AB - Whole brains dissected from third-instar larvae of Drosophila melanogaster increase their cAMP content when incubated with octopamine, dopamine and serotonin, especially in the presence of theophylline, a phosphodiesterase inhibitor. The most pronounced rise in cAMP was produced by forskolin. When brains prelabelled with [32P]orthophosphate were subjected to these treatments, the increased labelling of 27.5-kDa protein(s) of pi 6.3-6.6 (substrate A) was observed in all cases, as revealed by two-dimensional gel electrophoresis and autoradiography. Labelling of substrate A was enhanced without any drug addition in brains of the dunceM11 mutant strain, which has abnormally high basal cAMP levels. The labelling of substrate A was also promoted by the potassium channel blocker 4-aminopyridine. The data suggest that in Drosophila larval brain the 27.5-kDa protein is a target of cAMP-triggered phosphorylation and may be related to potassium channel(s).

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KW - Memory mutant dunce

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