Cutting edge: A new player in the alternative complement pathway, MASP-1 is essential for LPS-induced, but not for zymosan-induced, alternative pathway activation

Katalin Parej, Andrea Kocsis, Csenge Enyingi, Rahel Dani, Gabor Oroszlan, Laszlo Beinrohr, J. Dobó, P. Závodszky, G. Pál, P. Gál

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The complement system is a sophisticated network of proteases. In this article, we describe an unexpected link between two linear activation routes of the complement system: the lectin pathway (LP) and the alternative pathway (AP). Mannose-lectin binding-associated serine protease (MASP)-1 is known to be the initiator protease of the LP. Using a specific and potent inhibitor of MASP-1, SGMI-1, as well as other MASP-1 inhibitors with different mechanisms of action, we demonstrated that, in addition to its functions in the LP, MASP-1 is essential for bacterial LPS-induced AP activation, whereas it has little effect on zymosaninduced AP activation. We have shown that MASP-1 inhibition prevents AP activation, as well as attenuates the already initiated AP activity on the LPS surface. This newly recognized function of MASP-1 can be important for the defense against certain bacterial infections. Our results also emphasize that the mechanism of AP activation depends on the activator surface.

Original languageEnglish
Pages (from-to)2247-2252
Number of pages6
JournalJournal of Immunology
Volume200
Issue number7
DOIs
Publication statusPublished - Apr 1 2018

Fingerprint

Mannose-Binding Lectin
Alternative Complement Pathway
Zymosan
Serine Proteases
Lectins
Mannose-Binding Lectin Complement Pathway
Peptide Hydrolases
Serine Proteinase Inhibitors
Bacterial Infections

ASJC Scopus subject areas

  • Immunology

Cite this

Cutting edge : A new player in the alternative complement pathway, MASP-1 is essential for LPS-induced, but not for zymosan-induced, alternative pathway activation. / Parej, Katalin; Kocsis, Andrea; Enyingi, Csenge; Dani, Rahel; Oroszlan, Gabor; Beinrohr, Laszlo; Dobó, J.; Závodszky, P.; Pál, G.; Gál, P.

In: Journal of Immunology, Vol. 200, No. 7, 01.04.2018, p. 2247-2252.

Research output: Contribution to journalArticle

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