Cu2+ is the active principle of an endogenous substance from porcine cerebral cortex which antagonizes the anticonvulsant effect of diazepam

J. Kardos, J. Samu, K. Ujszászi, J. Nagy, I. Kovács, J. Visy, G. Maksay, M. Simonyi

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

A boiled extract of porcine cerebral cortex was fractionated on Sephadex G-75 and LH-20 followed by paper chromatography of the active fraction having inhibitory activity towards [3H]GABA binding to rat brain synaptic membranes. A ninhydrin-negative substance migrating more quickly than authentic GABA was identified as a copper-GABA complex. The complex inhibited specific [3H]GABA binding (IC50 ≈ 1 μM) and antagonized the anticonvulsant effect exerted by intraamygdaloid injection of diazepam. The effect of a synthetic copper-GABA complex was compared and found to be similar to the endogenous complex. Cu2+ alone has no affinity for the GABA recognition site but antagonizes the anticonvulsant effect of diazepam. Therefore, Cu2+ is suggested to be the pharmacologically active principle of the endogenous substance. Cu2+ does not seem to function via the recognition site of the GABA receptor.

Original languageEnglish
Pages (from-to)67-72
Number of pages6
JournalNeuroscience Letters
Volume52
Issue number1-2
DOIs
Publication statusPublished - Nov 23 1984

Fingerprint

Diazepam
Cerebral Cortex
Anticonvulsants
gamma-Aminobutyric Acid
Swine
Copper
Ninhydrin
Paper Chromatography
Synaptic Membranes
GABA Receptors
Inhibitory Concentration 50
Injections
Brain

Keywords

  • antagonism of anticonvulsant
  • cerebral cortex
  • copper
  • Cu
  • effect of diazepam
  • endogenous substance
  • GABA complex
  • porcine
  • [H]GABA binding

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Cu2+ is the active principle of an endogenous substance from porcine cerebral cortex which antagonizes the anticonvulsant effect of diazepam. / Kardos, J.; Samu, J.; Ujszászi, K.; Nagy, J.; Kovács, I.; Visy, J.; Maksay, G.; Simonyi, M.

In: Neuroscience Letters, Vol. 52, No. 1-2, 23.11.1984, p. 67-72.

Research output: Contribution to journalArticle

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