Current Trends in the Analysis of Post-translational Modifications

Research output: Contribution to journalReview article

Abstract

Post-translational modifications controlling a large number of biological functions are key aspects of protein diversity. They have an important role controlling cellular processes and may be advantageously utilized. Qualitative and quantitative analyses of post-translational modifications are useful for biomarker research and an integral part of the characterization of protein biopharmaceuticals. Due to its sensitivity and widespread applicability, mass spectrometry has become the core technology of the analysis especially when combined with chromatographic and other separation techniques. The aim of this article is to present a general overview of mass spectrometry applications in the field of PTM mapping. We also present the analytical challenges of particular PTMs, primarily focusing on the most frequent modifications.

Original languageEnglish
JournalChromatographia
Volume83
Issue number1
DOIs
Publication statusPublished - Jan 1 2020

    Fingerprint

Keywords

  • Liquid chromatography
  • Mass spectrometry
  • Post-translational modification
  • Protein

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Organic Chemistry

Cite this