Cultured tonsillar lymphocytes excrete [3H]thymidine labeled DNA and revert to resting state

M. Sasvári, M. Staub, Janos Taljanidisz, Gabor Szabo, Jozsef Takacs, Ferenc Antoni

Research output: Contribution to journalArticle

Abstract

Large tonsillar lymphocytes labeled with [3H]thymidine reverted to small lymphocytes with concomitant loss of [3H]DNA upon culturing. The decrease of labeled DNA content and size of large lymphocytes was demonstrated by flow cytometry and cell sorting. These observations suggest that stimulated lymphocytes may revert from their proliferative phase to resting phase by shedding 'extra'DNA under cell culture conditions. This released DNA is not due to cell damage and can be hybridized to chromosomal DNA.

Original languageEnglish
Pages (from-to)1110-1116
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume125
Issue number3
DOIs
Publication statusPublished - Dec 28 1984

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Lymphocytes
Thymidine
DNA
Cells
Flow cytometry
Sorting
Cell culture
Flow Cytometry
Cell Culture Techniques

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Cultured tonsillar lymphocytes excrete [3H]thymidine labeled DNA and revert to resting state. / Sasvári, M.; Staub, M.; Taljanidisz, Janos; Szabo, Gabor; Takacs, Jozsef; Antoni, Ferenc.

In: Biochemical and Biophysical Research Communications, Vol. 125, No. 3, 28.12.1984, p. 1110-1116.

Research output: Contribution to journalArticle

Sasvári, M. ; Staub, M. ; Taljanidisz, Janos ; Szabo, Gabor ; Takacs, Jozsef ; Antoni, Ferenc. / Cultured tonsillar lymphocytes excrete [3H]thymidine labeled DNA and revert to resting state. In: Biochemical and Biophysical Research Communications. 1984 ; Vol. 125, No. 3. pp. 1110-1116.
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