Culture of in vitro produced bovine zygotes in vitro vs in vivo: Implications for early embryo development and quality

B. P. Enright, P. Lonergan, A. Dinnyés, T. Fair, F. A. Ward, X. Yang, M. P. Boland

Research output: Contribution to journalArticle

138 Citations (Scopus)

Abstract

The objectives of this study were to examine the effect of culture system on bovine blastocyst formation rates and quality. Presumptive IVM/IVF bovine zygotes were cultured either in vitro in synthetic oviduct fluid (SOF, 25 embryos/25 μL in 5% CO2, 5% O2, 90% N2 at 39°°) or in vivo in the ewe oviduct (approximately 100 embryos per oviduct). The recovery rate after in vivo culture was 53% (813/1530). The blastocyst rate on Day 7 was significantly higher for the in vitro system (28%, 362/1278 vs 17%, 37/813; P

Original languageEnglish
Pages (from-to)659-673
Number of pages15
JournalTheriogenology
Volume54
Issue number5
DOIs
Publication statusPublished - Sep 15 2000

Fingerprint

Oviducts
Zygote
zygote
oviducts
in vitro culture
Embryonic Development
embryogenesis
Blastocyst
blastocyst
cattle
embryo (animal)
Embryonic Structures
ewes
In Vitro Techniques

Keywords

  • Ewe oviduct
  • Freezing
  • IVP
  • SOF
  • Vitrification

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)

Cite this

Culture of in vitro produced bovine zygotes in vitro vs in vivo : Implications for early embryo development and quality. / Enright, B. P.; Lonergan, P.; Dinnyés, A.; Fair, T.; Ward, F. A.; Yang, X.; Boland, M. P.

In: Theriogenology, Vol. 54, No. 5, 15.09.2000, p. 659-673.

Research output: Contribution to journalArticle

Enright, B. P. ; Lonergan, P. ; Dinnyés, A. ; Fair, T. ; Ward, F. A. ; Yang, X. ; Boland, M. P. / Culture of in vitro produced bovine zygotes in vitro vs in vivo : Implications for early embryo development and quality. In: Theriogenology. 2000 ; Vol. 54, No. 5. pp. 659-673.
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