Granulosa sejtek in vitro sejtkulturaja, mint az ovarialis mukodes modellje: A rekombinans gonadotropinok hatasainak vizsgalata a sejtek szteroid bioszintezisere

Translated title of the contribution: Culture of granulosa cells as a model for ovarian function: Evaluation of the effects of recombinant gonadotropins on steroid biosynthesis

I. Földesi, M. Breckwoldt, J. Neulen

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

This study was designed to investigate the effects of recombinant gonadotropins on steroid production of granulosa cells harvested at the time of follicle aspiration for in vitro fertilization. Cells secreted high amounts of progesterone and displayed high aromatase activity during the first days of culture. The individual variations in serum estrogen levels, measured on day of hCG injection for ovulation induction, were also reflected in differences in granulosa cell estrogen production when assayed within the first day of culture. All experiments were performed after 3 days pre- incubation. Addition of testosterone resulted in dose-dependent increases in estradiol production. Cells maintained their capacity to produce estradiol during the whole culture. By comparison, in spite of elevated baseline progesterone levels in the presence of increasing amounts of fetal calf serum (FCS), progesterone secretion decreased after the initial 3 days preincubation even in the presence of high FCS levels. In the absence of androgens, rFSH enhanced estradiol production in a dose-dependent manner. However, in the presence of testosterone, rFSH failed to alter estrogen production. Recombinant hCG significantly stimulated granulosa cell progesterone production. A decline was still noted in progesterone secretion towards the end of culture even in the presence of rhCG, but it was less pronounced than in the absence of rhCG. From these results it can be concluded that the in vitro progesterone production by granulosa cells collected from stimulated cycles appear to be dependent on both substrate availability and gonadotropin stimulation, whereas these cells possess high aromatase activity and are able to convert androgens to estrogens even without gonadotropin support.

Original languageHungarian
Pages (from-to)373-380
Number of pages8
JournalMagyar Noorvosok Lapja
Volume62
Issue number5
Publication statusPublished - 1999

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Granulosa Cells
Gonadotropins
Progesterone
Steroids
Estrogens
Estradiol
Aromatase
Androgens
Testosterone
Serum
Ovulation Induction
Fertilization in Vitro
Injections

ASJC Scopus subject areas

  • Obstetrics and Gynaecology

Cite this

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title = "Granulosa sejtek in vitro sejtkulturaja, mint az ovarialis mukodes modellje: A rekombinans gonadotropinok hatasainak vizsgalata a sejtek szteroid bioszintezisere",
abstract = "This study was designed to investigate the effects of recombinant gonadotropins on steroid production of granulosa cells harvested at the time of follicle aspiration for in vitro fertilization. Cells secreted high amounts of progesterone and displayed high aromatase activity during the first days of culture. The individual variations in serum estrogen levels, measured on day of hCG injection for ovulation induction, were also reflected in differences in granulosa cell estrogen production when assayed within the first day of culture. All experiments were performed after 3 days pre- incubation. Addition of testosterone resulted in dose-dependent increases in estradiol production. Cells maintained their capacity to produce estradiol during the whole culture. By comparison, in spite of elevated baseline progesterone levels in the presence of increasing amounts of fetal calf serum (FCS), progesterone secretion decreased after the initial 3 days preincubation even in the presence of high FCS levels. In the absence of androgens, rFSH enhanced estradiol production in a dose-dependent manner. However, in the presence of testosterone, rFSH failed to alter estrogen production. Recombinant hCG significantly stimulated granulosa cell progesterone production. A decline was still noted in progesterone secretion towards the end of culture even in the presence of rhCG, but it was less pronounced than in the absence of rhCG. From these results it can be concluded that the in vitro progesterone production by granulosa cells collected from stimulated cycles appear to be dependent on both substrate availability and gonadotropin stimulation, whereas these cells possess high aromatase activity and are able to convert androgens to estrogens even without gonadotropin support.",
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N2 - This study was designed to investigate the effects of recombinant gonadotropins on steroid production of granulosa cells harvested at the time of follicle aspiration for in vitro fertilization. Cells secreted high amounts of progesterone and displayed high aromatase activity during the first days of culture. The individual variations in serum estrogen levels, measured on day of hCG injection for ovulation induction, were also reflected in differences in granulosa cell estrogen production when assayed within the first day of culture. All experiments were performed after 3 days pre- incubation. Addition of testosterone resulted in dose-dependent increases in estradiol production. Cells maintained their capacity to produce estradiol during the whole culture. By comparison, in spite of elevated baseline progesterone levels in the presence of increasing amounts of fetal calf serum (FCS), progesterone secretion decreased after the initial 3 days preincubation even in the presence of high FCS levels. In the absence of androgens, rFSH enhanced estradiol production in a dose-dependent manner. However, in the presence of testosterone, rFSH failed to alter estrogen production. Recombinant hCG significantly stimulated granulosa cell progesterone production. A decline was still noted in progesterone secretion towards the end of culture even in the presence of rhCG, but it was less pronounced than in the absence of rhCG. From these results it can be concluded that the in vitro progesterone production by granulosa cells collected from stimulated cycles appear to be dependent on both substrate availability and gonadotropin stimulation, whereas these cells possess high aromatase activity and are able to convert androgens to estrogens even without gonadotropin support.

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