Crystallization of redox-insensitive Oct1 POU domain with different DNA-response elements

A. Reményi, E. Pohl, H. R. Schöler, M. Wilmanns

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The POU domain of the human Oct1 transcription factor has been crystallized with two different POU-dimer-binding DNA elements. Protein-DNA cocrystals suitable for structural analysis could be obtained only with a redox-insensitive version of the POU domain. The recombinant protein expression in a prokaryotic host was adjusted for fast purification. Optimized crystals were obtained by systematically varying the length of the oligonucleotide and by modifying cryofreezing procedures. These steps are generally applicable to the preparation of protein-DNA complexes for structural studies.

Original languageEnglish
Pages (from-to)1634-1638
Number of pages5
JournalActa Crystallographica Section D: Biological Crystallography
Volume57
Issue number11
DOIs
Publication statusPublished - Nov 20 2001

ASJC Scopus subject areas

  • Structural Biology

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