Crystallization and preliminary crystallographic analysis of dUTPase from the ψ11 helper phage of Staphylococcus aureus

Ibolya Leveles, Gergely Róna, Imre Zagyva, Ábris Bendes, V. Harmat, B. Vértessy

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Staphylococcus aureus superantigen-carrying pathogenicity islands (SaPIs) play a determinant role in spreading virulence genes among bacterial populations that constitute a major health hazard. Repressor (Stl) proteins are responsible for the transcriptional regulation of pathogenicity island genes. Recently, a derepressing interaction between the repressor Stl SaPIbov1 and dUTPase from the ψ11 helper phage has been suggested [Tormo-Más et al. (2010), Nature (London), 465, 779-782]. Towards elucidation of the molecular mechanism of this interaction, this study reports the expression, purification and X-ray analysis of ψ11 dUTPase, which contains a phage-specific polypeptide segment that is not present in other dUTPases. Crystals were obtained using the hanging-drop vapour-diffusion method at room temperature. Data were collected to 2.98 Å resolution from one type of crystal. The crystal of ψ11 dUTPase belonged to the cubic space group I23, with unit-cell parameters a = 98.16 Å, α = β = γ = 90.00°.

Original languageEnglish
Pages (from-to)1411-1413
Number of pages3
JournalActa Crystallographica Section F: Structural Biology and Crystallization Communications
Volume67
Issue number11
DOIs
Publication statusPublished - Nov 2011

Fingerprint

Bacteriophages
staphylococcus
Crystallization
Genomic Islands
Staphylococcus aureus
crystallization
genes
Crystals
Genes
virulence
Repressor Proteins
crystals
Bacterial Genes
Superantigens
Health hazards
polypeptides
X ray analysis
purification
determinants
hazards

Keywords

  • ψ11 helper phage
  • dUTPases

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Structural Biology
  • Genetics
  • Condensed Matter Physics

Cite this

Crystallization and preliminary crystallographic analysis of dUTPase from the ψ11 helper phage of Staphylococcus aureus. / Leveles, Ibolya; Róna, Gergely; Zagyva, Imre; Bendes, Ábris; Harmat, V.; Vértessy, B.

In: Acta Crystallographica Section F: Structural Biology and Crystallization Communications, Vol. 67, No. 11, 11.2011, p. 1411-1413.

Research output: Contribution to journalArticle

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