Cryopreservation of wheat (Triticum aestivum L.) egg cells by vitrification

Attila Fábián, Katalin Jäger, E. Darkó, B. Barnabás

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

A procedure has been developed for the cryopreservation of wheat female gametes. The procedure involves loading the cells with 25% concentrated vitrification solution consisting of 30% glycerol, 10% sucrose, 120 mM ascorbic acid (AA) and 5% propylene glycol (PG), dehydration in 80% concentrated vitrification solution, droplet vitrification and storage in liquid nitrogen, unloading and rehydration of the cells by gradual addition of isolation solution. Supplementation with AA significantly increased the proportion of viable egg cells after de- and rehydration. During the early phase of rehydration AA reduced the probability of membrane damage caused by rapid water uptake. Maintaining the temperature of the cells at 0°C during the de- and rehydration processes increased cell survival. Microscopic examination of the semi-thin sections of untreated and viable cryopreserved cells revealed that the vitrification process might cause changes in cell structure.

Original languageEnglish
Pages (from-to)737-744
Number of pages8
JournalActa Physiologiae Plantarum
Volume30
Issue number5
DOIs
Publication statusPublished - Sep 2008

Fingerprint

Vitrification
Cryopreservation
vitrification
rehydration
ova
cryopreservation
Triticum
Ovum
Triticum aestivum
Fluid Therapy
wheat
ascorbic acid
Dehydration
Ascorbic Acid
cells
propylene glycol
cell structures
water uptake
droplets
cell viability

Keywords

  • Cryopreservation
  • Dimethyl sulphoxide
  • Egg cell
  • Propylene glycol
  • Wheat

ASJC Scopus subject areas

  • Plant Science

Cite this

Cryopreservation of wheat (Triticum aestivum L.) egg cells by vitrification. / Fábián, Attila; Jäger, Katalin; Darkó, E.; Barnabás, B.

In: Acta Physiologiae Plantarum, Vol. 30, No. 5, 09.2008, p. 737-744.

Research output: Contribution to journalArticle

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