Cryopreservation of sperm of two European percid species, the pikeperch (Sander lucioperca) and the volga pikeperch (S. volgensis)

Z. Bokor, T. Müller, M. Bercsényi, L. Horváth, B. Urbányi, Á Horváth

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Experiments were carried out on sperm cryopreservation of two European percid fish species, the pikeperch (Sander lucioperca) and the Volga pikeperch (S. volgensis). Two experiments were conducted on pikeperch sperm. In the first, the effects of three extenders (Glucose, KCl, Sucrose) and two cryoprotectants (dimethyl-sulfoxide: DMSO, methanol: MeOH) were tested on motility and fertilization. In the second, the effects of two dilution ratios (1 : 1, 1 : 9) and two cryoprotectants (DMSO, MeOH) on hatching were investigated. In the experiment on Volga pikeperch the suitability of using cryopreservation for fertilization was investigated. In the first experiment on pikeperch the highest post-thaw motility (28 ± 21%) and fertilization rate (43 ± 12%) was found with DMSO as cryoprotectant in combination with Glucose extender. In the second, the highest hatch rate (41 ± 22%) was observed with MeOH as cryoprotectant and 1 : 1 sperm dilution ratio, however no significant difference was found among the results. In the experiment on Volga pikeperch hatch rates with cryopreserved sperm (60 ± 2%) did not significantly differ from the control (60 ± 6%). Contamination of sperm with urine seems to be a key problem in the success of sperm cryopreservation of these species.

Original languageEnglish
Pages (from-to)199-207
Number of pages9
JournalActa Biologica Hungarica
Volume58
Issue number2
DOIs
Publication statusPublished - Jun 2007

Fingerprint

percid
Sanders
cryopreservation
Sander lucioperca
Cryopreservation
sperm
Spermatozoa
Dimethyl Sulfoxide
spermatozoa
cryoprotectants
Hatches
fertilization (reproduction)
Fertilization
Experiments
Dilution
motility
experiment
glucose
dilution
Glucose

Keywords

  • Cryopreservation
  • Pikeperch
  • Sperm
  • Urine
  • Volga pikeperch

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

Cryopreservation of sperm of two European percid species, the pikeperch (Sander lucioperca) and the volga pikeperch (S. volgensis). / Bokor, Z.; Müller, T.; Bercsényi, M.; Horváth, L.; Urbányi, B.; Horváth, Á.

In: Acta Biologica Hungarica, Vol. 58, No. 2, 06.2007, p. 199-207.

Research output: Contribution to journalArticle

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abstract = "Experiments were carried out on sperm cryopreservation of two European percid fish species, the pikeperch (Sander lucioperca) and the Volga pikeperch (S. volgensis). Two experiments were conducted on pikeperch sperm. In the first, the effects of three extenders (Glucose, KCl, Sucrose) and two cryoprotectants (dimethyl-sulfoxide: DMSO, methanol: MeOH) were tested on motility and fertilization. In the second, the effects of two dilution ratios (1 : 1, 1 : 9) and two cryoprotectants (DMSO, MeOH) on hatching were investigated. In the experiment on Volga pikeperch the suitability of using cryopreservation for fertilization was investigated. In the first experiment on pikeperch the highest post-thaw motility (28 ± 21{\%}) and fertilization rate (43 ± 12{\%}) was found with DMSO as cryoprotectant in combination with Glucose extender. In the second, the highest hatch rate (41 ± 22{\%}) was observed with MeOH as cryoprotectant and 1 : 1 sperm dilution ratio, however no significant difference was found among the results. In the experiment on Volga pikeperch hatch rates with cryopreserved sperm (60 ± 2{\%}) did not significantly differ from the control (60 ± 6{\%}). Contamination of sperm with urine seems to be a key problem in the success of sperm cryopreservation of these species.",
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