Cryopreservation of common carp (Cyprinus carpio) sperm in 1.2 and 5 ml straws and occurrence of haploids among larvae produced with cryopreserved sperm

A. Horváth, Edit Miskolczi, Szilvia Mihálffy, Katalin Osz, Krisztián Szabó, B. Urbányi

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Experiments were carried out on the cryopreservation of common carp (Cyprinus carpio) sperm in order to test the suitability of using 1.2 and 5 ml straws and to investigate the ploidy of malformed larvae found among the hatched progeny. In the first set of experiments, the effect of freezing time was investigated on the hatch rate of embryos. The highest hatch rate for 1.2 ml straws was 69 ± 16% at the freezing time of 4 min, and 39 ± 27% for 5 ml straws at 5 min. In the second set, the effect different egg volumes fertilized with one straw of sperm on the hatch rate and the rate of malformed larvae was investigated. The highest hatch rate with 1.2 ml straws (86 ± 12%) was observed when 10 g of eggs were fertilized with one straw, whereas with 5 ml straws the hatch rate was highest (65 ± 18%) when 40 g of eggs were fertilized. The highest rate of malformed larvae (15 ± 9%) was found in the control, whereas the highest rate of malformed larvae among the groups fertilized with cryopreserved sperm (13 ± 7%) was found in the 1× dose group fertilized with 5 ml straw. The chromosome numbers of malformed larvae were investigated and haploids were found among those hatched from eggs fertilized with cryopreserved sperm whereas only diploids were found in the controls.

Original languageEnglish
Pages (from-to)251-257
Number of pages7
JournalCryobiology
Volume54
Issue number3
DOIs
Publication statusPublished - Jun 2007

Fingerprint

Carps
Haploidy
Cryopreservation
Straw
Cyprinus carpio
haploidy
cryopreservation
Zygote
Larva
Spermatozoa
straw
Hatches
spermatozoa
larvae
Freezing
Ploidies
freezing
Diploidy
Embryonic Structures
Chromosomes

Keywords

  • 1.2 ml Straws
  • 5 ml Straws
  • Chromosomes
  • Common carp
  • Cryopreservation
  • Diploids
  • Egg volume
  • Haploids
  • Larval malformation
  • Sperm

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

Cryopreservation of common carp (Cyprinus carpio) sperm in 1.2 and 5 ml straws and occurrence of haploids among larvae produced with cryopreserved sperm. / Horváth, A.; Miskolczi, Edit; Mihálffy, Szilvia; Osz, Katalin; Szabó, Krisztián; Urbányi, B.

In: Cryobiology, Vol. 54, No. 3, 06.2007, p. 251-257.

Research output: Contribution to journalArticle

@article{9d7068d93c574c8b9e90bcf8115f0704,
title = "Cryopreservation of common carp (Cyprinus carpio) sperm in 1.2 and 5 ml straws and occurrence of haploids among larvae produced with cryopreserved sperm",
abstract = "Experiments were carried out on the cryopreservation of common carp (Cyprinus carpio) sperm in order to test the suitability of using 1.2 and 5 ml straws and to investigate the ploidy of malformed larvae found among the hatched progeny. In the first set of experiments, the effect of freezing time was investigated on the hatch rate of embryos. The highest hatch rate for 1.2 ml straws was 69 ± 16{\%} at the freezing time of 4 min, and 39 ± 27{\%} for 5 ml straws at 5 min. In the second set, the effect different egg volumes fertilized with one straw of sperm on the hatch rate and the rate of malformed larvae was investigated. The highest hatch rate with 1.2 ml straws (86 ± 12{\%}) was observed when 10 g of eggs were fertilized with one straw, whereas with 5 ml straws the hatch rate was highest (65 ± 18{\%}) when 40 g of eggs were fertilized. The highest rate of malformed larvae (15 ± 9{\%}) was found in the control, whereas the highest rate of malformed larvae among the groups fertilized with cryopreserved sperm (13 ± 7{\%}) was found in the 1× dose group fertilized with 5 ml straw. The chromosome numbers of malformed larvae were investigated and haploids were found among those hatched from eggs fertilized with cryopreserved sperm whereas only diploids were found in the controls.",
keywords = "1.2 ml Straws, 5 ml Straws, Chromosomes, Common carp, Cryopreservation, Diploids, Egg volume, Haploids, Larval malformation, Sperm",
author = "A. Horv{\'a}th and Edit Miskolczi and Szilvia Mih{\'a}lffy and Katalin Osz and Kriszti{\'a}n Szab{\'o} and B. Urb{\'a}nyi",
year = "2007",
month = "6",
doi = "10.1016/j.cryobiol.2007.02.003",
language = "English",
volume = "54",
pages = "251--257",
journal = "Cryobiology",
issn = "0011-2240",
publisher = "Academic Press Inc.",
number = "3",

}

TY - JOUR

T1 - Cryopreservation of common carp (Cyprinus carpio) sperm in 1.2 and 5 ml straws and occurrence of haploids among larvae produced with cryopreserved sperm

AU - Horváth, A.

AU - Miskolczi, Edit

AU - Mihálffy, Szilvia

AU - Osz, Katalin

AU - Szabó, Krisztián

AU - Urbányi, B.

PY - 2007/6

Y1 - 2007/6

N2 - Experiments were carried out on the cryopreservation of common carp (Cyprinus carpio) sperm in order to test the suitability of using 1.2 and 5 ml straws and to investigate the ploidy of malformed larvae found among the hatched progeny. In the first set of experiments, the effect of freezing time was investigated on the hatch rate of embryos. The highest hatch rate for 1.2 ml straws was 69 ± 16% at the freezing time of 4 min, and 39 ± 27% for 5 ml straws at 5 min. In the second set, the effect different egg volumes fertilized with one straw of sperm on the hatch rate and the rate of malformed larvae was investigated. The highest hatch rate with 1.2 ml straws (86 ± 12%) was observed when 10 g of eggs were fertilized with one straw, whereas with 5 ml straws the hatch rate was highest (65 ± 18%) when 40 g of eggs were fertilized. The highest rate of malformed larvae (15 ± 9%) was found in the control, whereas the highest rate of malformed larvae among the groups fertilized with cryopreserved sperm (13 ± 7%) was found in the 1× dose group fertilized with 5 ml straw. The chromosome numbers of malformed larvae were investigated and haploids were found among those hatched from eggs fertilized with cryopreserved sperm whereas only diploids were found in the controls.

AB - Experiments were carried out on the cryopreservation of common carp (Cyprinus carpio) sperm in order to test the suitability of using 1.2 and 5 ml straws and to investigate the ploidy of malformed larvae found among the hatched progeny. In the first set of experiments, the effect of freezing time was investigated on the hatch rate of embryos. The highest hatch rate for 1.2 ml straws was 69 ± 16% at the freezing time of 4 min, and 39 ± 27% for 5 ml straws at 5 min. In the second set, the effect different egg volumes fertilized with one straw of sperm on the hatch rate and the rate of malformed larvae was investigated. The highest hatch rate with 1.2 ml straws (86 ± 12%) was observed when 10 g of eggs were fertilized with one straw, whereas with 5 ml straws the hatch rate was highest (65 ± 18%) when 40 g of eggs were fertilized. The highest rate of malformed larvae (15 ± 9%) was found in the control, whereas the highest rate of malformed larvae among the groups fertilized with cryopreserved sperm (13 ± 7%) was found in the 1× dose group fertilized with 5 ml straw. The chromosome numbers of malformed larvae were investigated and haploids were found among those hatched from eggs fertilized with cryopreserved sperm whereas only diploids were found in the controls.

KW - 1.2 ml Straws

KW - 5 ml Straws

KW - Chromosomes

KW - Common carp

KW - Cryopreservation

KW - Diploids

KW - Egg volume

KW - Haploids

KW - Larval malformation

KW - Sperm

UR - http://www.scopus.com/inward/record.url?scp=34248395056&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34248395056&partnerID=8YFLogxK

U2 - 10.1016/j.cryobiol.2007.02.003

DO - 10.1016/j.cryobiol.2007.02.003

M3 - Article

VL - 54

SP - 251

EP - 257

JO - Cryobiology

JF - Cryobiology

SN - 0011-2240

IS - 3

ER -