Cryopreservation of common carp (Cyprinus carpio L.) sperm II. Optimal conditions for fertilization

I. Magyary, B. Urbányi, L. Horváth

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Abstract

Experiments were carried out on the cryopreservation of common carp (Cyprinus carpio L.) sperm. Optimal conditions for fertilization including suitable medium and sperm:egg ratio were determined. Sperm was diluted in modified Kurokura's 'Extender 2' containing DMSO as cyroprotectant in 10% final concentration. The dilution rate was 1:9 (sperm:diluent). Sperm was diluted and equilibrated (10 min) at 2°C. Sperm was then frozen in plastic straws (0.5 ml) at the following rate: 0°C-4°C: 4°C min-1; -4°C-80°C: 11°C min-1; from -80°C they were plunged directly into liquid nitrogen (-196°C). Frozen samples were thawed in a water bath at 40°C. Fertilization rates achieved were much higher in water than in other solutions. Optimal ratios of frozen sperm:egg:water (1:20:20 in volume) and optimal number of frozen spermatozoa:egg (105 spz: 1 egg) were determined. In such conditions, a strong positive correlation (c = +0.846) was found between the post-thaw motility and the fertility of frozen sperm securing high fertilization (99.6%, percent of control). No significant difference was found between fertilization and hatching rates achieved using frozen-thawed common carp sperm.

Original languageEnglish
Pages (from-to)117-119
Number of pages3
JournalJournal of Applied Ichthyology
Volume12
Issue number2
DOIs
Publication statusPublished - Jul 1996

ASJC Scopus subject areas

  • Aquatic Science

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