Cryopreservation of Atlantic cod (Gadus morhua) sperm in large-volume straws: Applications for commercial production and gene banking

Ian A.E. Butts, Nathaniel Feindel, Steve Neil, Éva Kovács, Béla Urbányi, Edward A. Trippel

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

In our study, we used a full factorial analysis of variance design to examine the effects of diluent [Mounib's sucrose-based diluent+hen's egg yolk (EY) and Hanks' balanced salt solution (HBSS)+EY], freezing rate (-2.5, -5.0 and -7.5°Cmin -1) and thawing rate (2.5, 5.0 and 7.5°Cmin -1) on motility and velocity of Atlantic cod sperm cryopreserved in 2.5mL cryogenic straws. We found that post-thaw sperm performance was strongly influenced by the presence of higher-order interactions of the factors we tested. For all models broken down by diluent, the 2.5°Cmin -1 thawing rate had the lowest sperm motility recovery index. Mounib's sucrose-based diluent+EY had the highest motility recovery index at all thawing rates. Mean per cent motility for fresh sperm (87.7±2.9%) was not significantly different than of sperm cryopreserved using Mounib's sucrose-based diluent+EY, frozen at -2.5°Cmin -1 and thawed at 5.0°Cmin -1 (77.1±2.9%). For Mounib's sucrose-based diluent+EY, velocity was significantly higher with sperm thawed at 7.5°Cmin -1, than sperm thawed at 2.5°Cmin -1, while thawing rate had no effect for HBSS+EY. Our findings have implications for cod mariculture and aiding in conservation efforts for a dominant marine fish species.

Original languageEnglish
Pages (from-to)1714-1722
Number of pages9
JournalAquaculture Research
Volume42
Issue number11
DOIs
Publication statusPublished - Oct 1 2011

Keywords

  • Atlantic cod
  • Cryopreservation
  • Motility
  • Sperm
  • Thaw
  • Velocity

ASJC Scopus subject areas

  • Aquatic Science

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