Crosslinking of troponin complex with 1,3-difluoro-4,6-dinitrobenzene. Identification of the crosslink formed between troponin C and troponin I in the absence of Ca2+

Anatoly B. Dobrovol'sky; Nikolay B. Gusev; P. Friedrich

doi:10.1016/0167-4838(84)90198-5

Crosslinking of troponin complex with 1,3-difluoro-4,6-dinitrobenzene. Identification of the crosslink formed between troponin C and troponin I in the absence of Ca²⁺

Anatoly B. Dobrovol'sky, Nikolay B. Gusev, P. Friedrich

Hungarian Academy of Sciences

Research output: Contribution to journal › Article

10 Citations (Scopus)

Abstract

The single SH-group of rabbit skeletal muscle troponin C (Cys-98) was reacted with the bifunctional reagent, 1,3-difluoro-4,6-dinitrobenzene. This labelled troponin C was used to reconstitute the troponin complex by the addition of equimolar amounts of troponin T and troponin I. The second function of the bifunctional reagent was triggered in the complex by an increase of pH. A crosslink was formed between troponin C and troponin I both in the presence and absence of Ca²⁺, but the probability of crosslinking was decreased by Ca²⁺. Covalently linked troponin C-trophonin I was isolated from the complex crosslinked without Ca²⁺, and cleaved by CNBr. The analysis of crosslinked peptides has revealed that in the presence of Mg²⁺ and absence of Ca²⁺ the crosslink in the troponin complex is formed between Cys-98 of troponin C and Cys-133 of troponin I.

Original language	English
Pages (from-to)	144-151
Number of pages	8
Journal	Biochimica et Biophysica Acta (BBA)/Protein Structure and Molecular
Volume	789
Issue number	2
DOIs	https://doi.org/10.1016/0167-4838(84)90198-5
Publication status	Published - Sep 11 1984

Fingerprint

Troponin C

Troponin

Troponin I

Crosslinking

Cross-Linking Reagents

Troponin T

Muscle

Skeletal Muscle

1,5-difluoro-2,4-dinitrobenzene

Rabbits

Peptides

Keywords

(Rabbit skeletal muscle)
Peptide analysis
Protein crosslinking
Reconstitution
Troponin

ASJC Scopus subject areas

Biochemistry
Biophysics
Molecular Biology
Structural Biology

Cite this

Dobrovol'sky, A. B., Gusev, N. B., & Friedrich, P. (1984). Crosslinking of troponin complex with 1,3-difluoro-4,6-dinitrobenzene. Identification of the crosslink formed between troponin C and troponin I in the absence of Ca²⁺. Biochimica et Biophysica Acta (BBA)/Protein Structure and Molecular, 789(2), 144-151. https://doi.org/10.1016/0167-4838(84)90198-5

Crosslinking of troponin complex with 1,3-difluoro-4,6-dinitrobenzene. Identification of the crosslink formed between troponin C and troponin I in the absence of Ca²⁺. / Dobrovol'sky, Anatoly B.; Gusev, Nikolay B.; Friedrich, P.

In: Biochimica et Biophysica Acta (BBA)/Protein Structure and Molecular, Vol. 789, No. 2, 11.09.1984, p. 144-151.

Research output: Contribution to journal › Article

Dobrovol'sky, AB, Gusev, NB & Friedrich, P 1984, 'Crosslinking of troponin complex with 1,3-difluoro-4,6-dinitrobenzene. Identification of the crosslink formed between troponin C and troponin I in the absence of Ca²⁺', Biochimica et Biophysica Acta (BBA)/Protein Structure and Molecular, vol. 789, no. 2, pp. 144-151. https://doi.org/10.1016/0167-4838(84)90198-5

Dobrovol'sky AB, Gusev NB, Friedrich P. Crosslinking of troponin complex with 1,3-difluoro-4,6-dinitrobenzene. Identification of the crosslink formed between troponin C and troponin I in the absence of Ca²⁺. Biochimica et Biophysica Acta (BBA)/Protein Structure and Molecular. 1984 Sep 11;789(2):144-151. https://doi.org/10.1016/0167-4838(84)90198-5

Dobrovol'sky, Anatoly B. ; Gusev, Nikolay B. ; Friedrich, P. / Crosslinking of troponin complex with 1,3-difluoro-4,6-dinitrobenzene. Identification of the crosslink formed between troponin C and troponin I in the absence of Ca²⁺. In: Biochimica et Biophysica Acta (BBA)/Protein Structure and Molecular. 1984 ; Vol. 789, No. 2. pp. 144-151.

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10.1016/0167-4838(84)90198-5