Abstract
The single SH-group of rabbit skeletal muscle troponin C (Cys-98) was reacted with the bifunctional reagent, 1,3-difluoro-4,6-dinitrobenzene. This labelled troponin C was used to reconstitute the troponin complex by the addition of equimolar amounts of troponin T and troponin I. The second function of the bifunctional reagent was triggered in the complex by an increase of pH. A crosslink was formed between troponin C and troponin I both in the presence and absence of Ca2+, but the probability of crosslinking was decreased by Ca2+. Covalently linked troponin C-trophonin I was isolated from the complex crosslinked without Ca2+, and cleaved by CNBr. The analysis of crosslinked peptides has revealed that in the presence of Mg2+ and absence of Ca2+ the crosslink in the troponin complex is formed between Cys-98 of troponin C and Cys-133 of troponin I.
Original language | English |
---|---|
Pages (from-to) | 144-151 |
Number of pages | 8 |
Journal | Biochimica et Biophysica Acta (BBA)/Protein Structure and Molecular |
Volume | 789 |
Issue number | 2 |
DOIs | |
Publication status | Published - Sep 11 1984 |
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Keywords
- (Rabbit skeletal muscle)
- Peptide analysis
- Protein crosslinking
- Reconstitution
- Troponin
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
- Structural Biology
Cite this
Crosslinking of troponin complex with 1,3-difluoro-4,6-dinitrobenzene. Identification of the crosslink formed between troponin C and troponin I in the absence of Ca2+. / Dobrovol'sky, Anatoly B.; Gusev, Nikolay B.; Friedrich, P.
In: Biochimica et Biophysica Acta (BBA)/Protein Structure and Molecular, Vol. 789, No. 2, 11.09.1984, p. 144-151.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Crosslinking of troponin complex with 1,3-difluoro-4,6-dinitrobenzene. Identification of the crosslink formed between troponin C and troponin I in the absence of Ca2+
AU - Dobrovol'sky, Anatoly B.
AU - Gusev, Nikolay B.
AU - Friedrich, P.
PY - 1984/9/11
Y1 - 1984/9/11
N2 - The single SH-group of rabbit skeletal muscle troponin C (Cys-98) was reacted with the bifunctional reagent, 1,3-difluoro-4,6-dinitrobenzene. This labelled troponin C was used to reconstitute the troponin complex by the addition of equimolar amounts of troponin T and troponin I. The second function of the bifunctional reagent was triggered in the complex by an increase of pH. A crosslink was formed between troponin C and troponin I both in the presence and absence of Ca2+, but the probability of crosslinking was decreased by Ca2+. Covalently linked troponin C-trophonin I was isolated from the complex crosslinked without Ca2+, and cleaved by CNBr. The analysis of crosslinked peptides has revealed that in the presence of Mg2+ and absence of Ca2+ the crosslink in the troponin complex is formed between Cys-98 of troponin C and Cys-133 of troponin I.
AB - The single SH-group of rabbit skeletal muscle troponin C (Cys-98) was reacted with the bifunctional reagent, 1,3-difluoro-4,6-dinitrobenzene. This labelled troponin C was used to reconstitute the troponin complex by the addition of equimolar amounts of troponin T and troponin I. The second function of the bifunctional reagent was triggered in the complex by an increase of pH. A crosslink was formed between troponin C and troponin I both in the presence and absence of Ca2+, but the probability of crosslinking was decreased by Ca2+. Covalently linked troponin C-trophonin I was isolated from the complex crosslinked without Ca2+, and cleaved by CNBr. The analysis of crosslinked peptides has revealed that in the presence of Mg2+ and absence of Ca2+ the crosslink in the troponin complex is formed between Cys-98 of troponin C and Cys-133 of troponin I.
KW - (Rabbit skeletal muscle)
KW - Peptide analysis
KW - Protein crosslinking
KW - Reconstitution
KW - Troponin
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UR - http://www.scopus.com/inward/citedby.url?scp=0021205335&partnerID=8YFLogxK
U2 - 10.1016/0167-4838(84)90198-5
DO - 10.1016/0167-4838(84)90198-5
M3 - Article
C2 - 6477927
AN - SCOPUS:0021205335
VL - 789
SP - 144
EP - 151
JO - Biochimica et Biophysica Acta - Proteins and Proteomics
JF - Biochimica et Biophysica Acta - Proteins and Proteomics
SN - 1570-9639
IS - 2
ER -