Cross-linking of CD32 induces maturation of human monocyte-derived dendritic cells via NF-κB signaling pathway

Zoltán Bánki, Laco Kacani, Brigitte Müllauer, Doris Wilflingseder, Gerlinde Obermoser, Harald Niederegger, Harald Schennach, Georg M. Sprinzl, Norbert Sepp, A. Erdei, Manfred P. Dierich, Heribert Stoiber

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Abstract

Dendritic cells (DC) represent a unique set of APCs that initiate immune responses through priming of naive T cells. Maturation of DC is a crucial step during Ag presentation and can be induced by triggering a broad spectrum of DC surface receptors. Although human DC express several receptors for the Fc portion of IgG which were described to play an important role in Ag internalization, little is known about the effects of IgG or immune complexes on DC maturation. In this study, we show that cross-linking of FcγR-type II (CD32) with immobilized IgG (imIgG) can induce maturation of human monocyte-derived DC via the NF-κB signaling pathway. IgG-mediated maturation was accompanied by a moderate increase of IL-10 secretion, whereas no IL-12 production was observed. Involvement of CD32 was further supported by experiments with the anti-CD32 mAb, which blocked IgG-triggered DC maturation and cytokine secretion significantly. Furthermore, DC cultivated in the presence of imIgG induced allogeneic T cell proliferation. Because this imIgG-induced maturation was considerably impaired in monocyte-derived DC from systemic lupus erythematosus patients, we suggest that DC, which matured in the presence of immune complexes, may contribute to prevention of pathological immune responses.

Original languageEnglish
Pages (from-to)3963-3970
Number of pages8
JournalJournal of Immunology
Volume170
Issue number8
Publication statusPublished - Apr 15 2003

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Dendritic Cells
Monocytes
Antigen-Antibody Complex
Immunoglobulin G
T-Lymphocytes
Fc Receptors
Cell Surface Receptors
Interleukin-12
Systemic Lupus Erythematosus
Interleukin-10
Cell Proliferation
Cytokines

ASJC Scopus subject areas

  • Immunology

Cite this

Bánki, Z., Kacani, L., Müllauer, B., Wilflingseder, D., Obermoser, G., Niederegger, H., ... Stoiber, H. (2003). Cross-linking of CD32 induces maturation of human monocyte-derived dendritic cells via NF-κB signaling pathway. Journal of Immunology, 170(8), 3963-3970.

Cross-linking of CD32 induces maturation of human monocyte-derived dendritic cells via NF-κB signaling pathway. / Bánki, Zoltán; Kacani, Laco; Müllauer, Brigitte; Wilflingseder, Doris; Obermoser, Gerlinde; Niederegger, Harald; Schennach, Harald; Sprinzl, Georg M.; Sepp, Norbert; Erdei, A.; Dierich, Manfred P.; Stoiber, Heribert.

In: Journal of Immunology, Vol. 170, No. 8, 15.04.2003, p. 3963-3970.

Research output: Contribution to journalArticle

Bánki, Z, Kacani, L, Müllauer, B, Wilflingseder, D, Obermoser, G, Niederegger, H, Schennach, H, Sprinzl, GM, Sepp, N, Erdei, A, Dierich, MP & Stoiber, H 2003, 'Cross-linking of CD32 induces maturation of human monocyte-derived dendritic cells via NF-κB signaling pathway', Journal of Immunology, vol. 170, no. 8, pp. 3963-3970.
Bánki Z, Kacani L, Müllauer B, Wilflingseder D, Obermoser G, Niederegger H et al. Cross-linking of CD32 induces maturation of human monocyte-derived dendritic cells via NF-κB signaling pathway. Journal of Immunology. 2003 Apr 15;170(8):3963-3970.
Bánki, Zoltán ; Kacani, Laco ; Müllauer, Brigitte ; Wilflingseder, Doris ; Obermoser, Gerlinde ; Niederegger, Harald ; Schennach, Harald ; Sprinzl, Georg M. ; Sepp, Norbert ; Erdei, A. ; Dierich, Manfred P. ; Stoiber, Heribert. / Cross-linking of CD32 induces maturation of human monocyte-derived dendritic cells via NF-κB signaling pathway. In: Journal of Immunology. 2003 ; Vol. 170, No. 8. pp. 3963-3970.
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AU - Obermoser, Gerlinde

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