Crk5 protein kinase contributes to the progression of embryogenesis of arabidopsis thaliana

Abu Imran Baba, Ildikó Valkai, Nitin M. Labhane, Lilla Koczka, Norbert Andrási, Éva Klement, Zsuzsanna Darula, Katalin F. Medzihradszky, László Szabados, Attila Fehér, Gábor Rigó, Ágnes Cséplő

Research output: Contribution to journalArticle

Abstract

The fine tuning of hormone (e.g., auxin and gibberellin) levels and hormone signaling is required for maintaining normal embryogenesis. Embryo polarity, for example, is ensured by the directional movement of auxin that is controlled by various types of auxin transporters. Here, we present pieces of evidence for the auxin-gibberellic acid (GA) hormonal crosstalk during embryo development and the regulatory role of the Arabidopsis thaliana Calcium-Dependent Protein Kinase-Related Kinase 5 (AtCRK5) in this regard. It is pointed out that the embryogenesis of the Atcrk5-1 mutant is delayed in comparison to the wild type. This delay is accompanied with a decrease in the levels of GA and auxin, as well as the abundance of the polar auxin transport (PAT) proteins PIN1, PIN4, and PIN7 in the mutant embryos. We have previously showed that AtCRK5 can regulate the PIN2 and PIN3 proteins either directly by phosphorylation or indirectly affecting the GA level during the root gravitropic and hypocotyl hook bending responses. In this manuscript, we provide evidence that the AtCRK5 protein kinase can in vitro phosphorylate the hydrophilic loops of additional PIN proteins that are important for embryogenesis. We propose that AtCRK5 can govern embryo development in Arabidopsis through the fine tuning of auxin-GA level and the accumulation of certain polar auxin transport proteins.

Original languageEnglish
Article number6120
JournalInternational journal of molecular sciences
Volume20
Issue number24
DOIs
Publication statusPublished - Dec 2 2019

Keywords

  • Arabidopsis thaliana
  • Auxin gradient
  • Calcium-Dependent Protein Kinase-Related Kinase (CRK)
  • Embryogenesis
  • GA3
  • Polar auxin transport (PAT) proteins

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

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  • Cite this

    Baba, A. I., Valkai, I., Labhane, N. M., Koczka, L., Andrási, N., Klement, É., Darula, Z., Medzihradszky, K. F., Szabados, L., Fehér, A., Rigó, G., & Cséplő, Á. (2019). Crk5 protein kinase contributes to the progression of embryogenesis of arabidopsis thaliana. International journal of molecular sciences, 20(24), [6120]. https://doi.org/10.3390/ijms20246120