CRF-stimulated biosynthesis of ACTH in a cell-free system from rat anterior pituitaries

Hiroshi Ishikawa, S. Goto

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

A cell-free system consisting of ribosomes, pH 5 enzymes and supernatant prepared from rat anterior pituitaries was found to be active in the incorporation of 3H-serine into ACTH. The rate of biosyntesis of ACTH, in a cell-free system as, measured by the incorporation of radioactive amino acid, and the rate of biological activity were markedly increased by the addition of CRF. The synthesis of ACTH was significantly inhibited by puromycin and RNAase but was not significantly inhibited by actinomycin D and DNAase.

Original languageEnglish
Pages (from-to)884-889
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume52
Issue number3
DOIs
Publication statusPublished - Jun 8 1973

Fingerprint

Cell-Free System
Biosynthesis
Adrenocorticotropic Hormone
Rats
Puromycin
Deoxyribonucleases
Dactinomycin
Bioactivity
Ribosomes
Serine
Amino Acids
Enzymes

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

CRF-stimulated biosynthesis of ACTH in a cell-free system from rat anterior pituitaries. / Ishikawa, Hiroshi; Goto, S.

In: Biochemical and Biophysical Research Communications, Vol. 52, No. 3, 08.06.1973, p. 884-889.

Research output: Contribution to journalArticle

@article{1fbf6a822e084b3d9d42d23992d77d29,
title = "CRF-stimulated biosynthesis of ACTH in a cell-free system from rat anterior pituitaries",
abstract = "A cell-free system consisting of ribosomes, pH 5 enzymes and supernatant prepared from rat anterior pituitaries was found to be active in the incorporation of 3H-serine into ACTH. The rate of biosyntesis of ACTH, in a cell-free system as, measured by the incorporation of radioactive amino acid, and the rate of biological activity were markedly increased by the addition of CRF. The synthesis of ACTH was significantly inhibited by puromycin and RNAase but was not significantly inhibited by actinomycin D and DNAase.",
author = "Hiroshi Ishikawa and S. Goto",
year = "1973",
month = "6",
day = "8",
doi = "10.1016/0006-291X(73)91020-6",
language = "English",
volume = "52",
pages = "884--889",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "3",

}

TY - JOUR

T1 - CRF-stimulated biosynthesis of ACTH in a cell-free system from rat anterior pituitaries

AU - Ishikawa, Hiroshi

AU - Goto, S.

PY - 1973/6/8

Y1 - 1973/6/8

N2 - A cell-free system consisting of ribosomes, pH 5 enzymes and supernatant prepared from rat anterior pituitaries was found to be active in the incorporation of 3H-serine into ACTH. The rate of biosyntesis of ACTH, in a cell-free system as, measured by the incorporation of radioactive amino acid, and the rate of biological activity were markedly increased by the addition of CRF. The synthesis of ACTH was significantly inhibited by puromycin and RNAase but was not significantly inhibited by actinomycin D and DNAase.

AB - A cell-free system consisting of ribosomes, pH 5 enzymes and supernatant prepared from rat anterior pituitaries was found to be active in the incorporation of 3H-serine into ACTH. The rate of biosyntesis of ACTH, in a cell-free system as, measured by the incorporation of radioactive amino acid, and the rate of biological activity were markedly increased by the addition of CRF. The synthesis of ACTH was significantly inhibited by puromycin and RNAase but was not significantly inhibited by actinomycin D and DNAase.

UR - http://www.scopus.com/inward/record.url?scp=0015917563&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0015917563&partnerID=8YFLogxK

U2 - 10.1016/0006-291X(73)91020-6

DO - 10.1016/0006-291X(73)91020-6

M3 - Article

C2 - 4351050

AN - SCOPUS:0015917563

VL - 52

SP - 884

EP - 889

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 3

ER -