CRF-stimulated biosynthesis of ACTH in a cell-free system from rat anterior pituitaries

Hiroshi Ishikawa, Sataro Goto

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1 Citation (Scopus)


A cell-free system consisting of ribosomes, pH 5 enzymes and supernatant prepared from rat anterior pituitaries was found to be active in the incorporation of 3H-serine into ACTH. The rate of biosyntesis of ACTH, in a cell-free system as, measured by the incorporation of radioactive amino acid, and the rate of biological activity were markedly increased by the addition of CRF. The synthesis of ACTH was significantly inhibited by puromycin and RNAase but was not significantly inhibited by actinomycin D and DNAase.

Original languageEnglish
Pages (from-to)884-889
Number of pages6
JournalBiochemical and biophysical research communications
Issue number3
Publication statusPublished - Jun 8 1973


ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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