Creation of a three-dimensional spherical fluorescence spot for super-resolution microscopy using a two-color annular hybrid wave plate

Yoshinori Iketaki, Hiroshi Kumagai, Kornel Jahn, Nandor Bokor

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

We fabricate a two-color annular hybrid wave plate (TAHWP) for fluorescence depletion super-resolution microscopy by combining quartz plates. Inserting the TAHWP into a commercial laser-scanning microscope together with a quarter-wave plate creates a three-dimensional spherical fluorescence spot smaller than the diffraction limit. Since a simple experimental setup easily provides a spot with a volume of 7sim;100 nm3, the TAHWP can improve the performance of various microspectroscopic devices.

Original languageEnglish
Pages (from-to)1057-1060
Number of pages4
JournalOptics Letters
Volume40
Issue number6
DOIs
Publication statusPublished - Mar 15 2015

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

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