We fabricate a two-color annular hybrid wave plate (TAHWP) for fluorescence depletion super-resolution microscopy by combining quartz plates. Inserting the TAHWP into a commercial laser-scanning microscope together with a quarter-wave plate creates a three-dimensional spherical fluorescence spot smaller than the diffraction limit. Since a simple experimental setup easily provides a spot with a volume of 7sim;100 nm3, the TAHWP can improve the performance of various microspectroscopic devices.
ASJC Scopus subject areas
- Atomic and Molecular Physics, and Optics