CR3 is the dominant phagocytotic complement receptor on human dendritic cells

Noémi Sándor, Katalin Kristóf, Katalin Paréj, Domonkos Pap, A. Erdei, Z. Bajtay

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Dendritic cells (DCs) play a decisive role in immunity; they interact with various pathogens via several pattern recognition and different opsonophagocytotic receptors, including Fc- and complement-receptors β2-integrins, including complement receptors CR3 (CD11b/CD18) and CR4 (CD11c/CD18) participate in many immunological processes, especially those involving cell migration, adherence, and phagocytosis. Human monocyte derived dendritic cells (MDCs) are known to express CR3 as well as CR4, however possible differences regarding the role of these receptors has not been addressed so far. Our aim was to explore whether there is a difference between the binding and uptake of various complement-opsonized microorganisms, mediated by CR3 and CR4. Studying the expression of receptors during differentiation of MDCs we found that the appearance of CD11b decreased, whereas that of CD11c increased. Interestingly, both receptors were present in the cell membrane in an active conformation. Here we demonstrate that ligation of CD11b directs MDCs to enhanced phagocytosis, while the maturation of the cells and their inflammatory cytokine production are not affected. Blocking CD11c alone did not change the uptake of opsonized yeast or bacteria by MDCs. We confirmed these results using siRNA; namely downregulation of CD11b blocked the phagocytosis of microbes while silencing CD11c had no effect on their uptake. Our data clearly demonstrate that complement C3-dependent phagocytosis of MDCs is mediated mainly by CR3.

Original languageEnglish
Pages (from-to)652-663
Number of pages12
JournalImmunobiology
Volume218
Issue number4
DOIs
Publication statusPublished - Apr 2013

Fingerprint

Complement Receptors
Dendritic Cells
Monocytes
Phagocytosis
Complement 3d Receptors
Cytophagocytosis
Complement C3
Fc Receptors
Integrins
Small Interfering RNA
Cell Movement
Ligation
Immunity
Down-Regulation
Yeasts
Cell Membrane
Cytokines
Bacteria

Keywords

  • β2-Integrins
  • CD11b/CD18
  • CD11c/CD18
  • Complement C3
  • Human dendritic cell
  • Phagocytosis

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Hematology

Cite this

CR3 is the dominant phagocytotic complement receptor on human dendritic cells. / Sándor, Noémi; Kristóf, Katalin; Paréj, Katalin; Pap, Domonkos; Erdei, A.; Bajtay, Z.

In: Immunobiology, Vol. 218, No. 4, 04.2013, p. 652-663.

Research output: Contribution to journalArticle

Sándor, Noémi ; Kristóf, Katalin ; Paréj, Katalin ; Pap, Domonkos ; Erdei, A. ; Bajtay, Z. / CR3 is the dominant phagocytotic complement receptor on human dendritic cells. In: Immunobiology. 2013 ; Vol. 218, No. 4. pp. 652-663.
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