CpG methylation in human papillomavirus (HPV) type 31 long control region (LCR) in cervical infections associated with cytological abnormalities

Brigitta László, Annamária Ferenczi, László Madar, Eszter Gyöngyösi, Anita Szalmás, Levente Szakács, G. Veress, József Kónya

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2 Citations (Scopus)

Abstract

The mechanisms that regulate papillomavirus gene expression include DNA methylation. The transcription of papillomavirus oncogenes E6 and E7 is controlled by certain regulatory elements in the LCR, which include binding sites for the E2 protein, a viral regulator of oncogene expression. In HPV-31-infected exfoliated cervical cells, the CpG methylation of the entire LCR was determined by next-generation sequencing after bisulfite modification. Six of the 22 cases had methylated CpG sites in the HPV-31 LCR, including position 7479 and/or 7485, at the promoter distal E2 binding site, thus suggesting a potential regulatory mechanism for papillomavirus transcription.

Original languageEnglish
Pages (from-to)1-4
Number of pages4
JournalVirus Genes
DOIs
Publication statusAccepted/In press - Apr 20 2016

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Keywords

  • CpG methylation
  • E2 binding site
  • HPV 31
  • Long control region (LCR)

ASJC Scopus subject areas

  • Virology
  • Genetics
  • Molecular Biology

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