Course and regression of acute interstitial pancreatitis induced in rats by repeated serial subcutaneous cholecystokinin-octapeptide injections

G. Zoltán Lászik, Zoltán Berger, A. Pap, G. Tóth, Vince Varró

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The aim of this study was to examine histologic and biochemical alterations in experimental acute interstitial pancreatitis (AIP) induced by serial repeated supramaximal cholecystokinin-octapeptide (CCK-OP) stimulation in rats. High doses of CCK-OP (60 μg/kg body wt) were administered subcutaneoulsy (sc) six times at hourly intervals for 1 d (Group I) or for 3, 5, or 7 d (Group II). Rats were killed after 1, 3, 5, 7, and 10 d in both groups and also after 13, 20, and 27 d in Group II. During the course of the AIP, the morphological alterations were more pronounced in the repeatedly treated rats, but their appearance and disappearance essentially occurred in parallel in the two groups. Increased mitotic- activity of the centroacinar and acinar cells was observed in d 5 and rose further even in Group II. The pancreatic weight and the protein and DNA contents reached a minimum on d 5 in both groups. The lowest enzyme activities did not occur in parallel. Thereafter, functional regeneration occurred despite continuing CCK-OP overstimulation in Group II. The toxicity of repeated CCK-OP hyper stimulation, thus, was limited: after its fifth administration, it failed to further aggravate the acute pancreatic damage or prevent the regeneration. This might be explained by a decreased CCK-OP sensitivity of the preexisting acinar cells, and/or increased CCK-OP tolerance of newly-formed ones.

Original languageEnglish
Pages (from-to)347-358
Number of pages12
JournalInternational Journal of Pancreatology
Volume5
Issue number4
DOIs
Publication statusPublished - Dec 1989

Fingerprint

Sincalide
Pancreatitis
Injections
Acinar Cells
Regeneration
Weights and Measures
DNA
Enzymes
Proteins

Keywords

  • cholecystokinin-octapeptide (CCK-OP)
  • DNA
  • Pancreatitis

ASJC Scopus subject areas

  • Oncology
  • Gastroenterology
  • Endocrinology

Cite this

Course and regression of acute interstitial pancreatitis induced in rats by repeated serial subcutaneous cholecystokinin-octapeptide injections. / Lászik, G. Zoltán; Berger, Zoltán; Pap, A.; Tóth, G.; Varró, Vince.

In: International Journal of Pancreatology, Vol. 5, No. 4, 12.1989, p. 347-358.

Research output: Contribution to journalArticle

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