Coupling immunomagnetic separation on magnetic beads with matrix-assisted laser desorption ionization-time of flight mass spectrometry for detection of staphylococcal enterotoxin B

G. Schlosser, Petr Kačer, Marek Kuzma, Zoltán Szilágyi, Alida Sorrentino, Carla Manzo, Rosa Pizzano, Livia Malorni, Gabriella Pocsfalvi

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

The growing importance of mass spectrometry for the identification and characterization of bacterial protein toxins is a consequence of the improved sensitivity and specificity of mass spectrometry-based techniques, especially when these techniques are combined with affinity methods. Here we describe a novel method based on the use of immunoaffinity capture and matrix-assisted laser desorption ionization-time of flight mass spectrometry for selective purification and detection of staphylococcal enterotoxin B (SEB). SEB is a potent bacterial protein toxin responsible for food poisoning, as well as a potential biological warfare agent. Unambiguous detection of SEB at low-nanogram levels in complex matrices is thus an important objective. In this work, an affinity molecular probe was prepared by immobilizing anti-SEB antibody on the surface of para-toluene-sulfonyl-functionalized monodisperse magnetic particles and used to selectively isolate SEB. Immobilization and affinity capture procedures were optimized to maximize the density of anti-SEB immunoglobulin G and the amount of captured SEB, respectively, on the surface of magnetic beads. SEB could be detected directly "on beads" by placing the molecular probe on the matrix-assisted laser desorption ionization target plate or, alternatively, "off beads" after its acidic elution. Application of this method to complex biological matrices was demonstrated by selective detection of SEB present in different matrices, such as cultivation media of Staphylococcus aureus strains and raw milk samples.

Original languageEnglish
Pages (from-to)6945-6952
Number of pages8
JournalApplied and Environmental Microbiology
Volume73
Issue number21
DOIs
Publication statusPublished - Nov 2007

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Immunomagnetic Separation
immunomagnetic separation
matrix-assisted laser desorption-ionization mass spectrometry
enterotoxins
Mass Spectrometry
desorption
Lasers
ionization
mass spectrometry
laser
matrix
toxin
Bacterial Toxins
probe
food poisoning
Molecular Probes
bacterial proteins
Bacterial Proteins
protein
probes (equipment)

ASJC Scopus subject areas

  • Environmental Science(all)
  • Biotechnology
  • Microbiology

Cite this

Coupling immunomagnetic separation on magnetic beads with matrix-assisted laser desorption ionization-time of flight mass spectrometry for detection of staphylococcal enterotoxin B. / Schlosser, G.; Kačer, Petr; Kuzma, Marek; Szilágyi, Zoltán; Sorrentino, Alida; Manzo, Carla; Pizzano, Rosa; Malorni, Livia; Pocsfalvi, Gabriella.

In: Applied and Environmental Microbiology, Vol. 73, No. 21, 11.2007, p. 6945-6952.

Research output: Contribution to journalArticle

Schlosser, G. ; Kačer, Petr ; Kuzma, Marek ; Szilágyi, Zoltán ; Sorrentino, Alida ; Manzo, Carla ; Pizzano, Rosa ; Malorni, Livia ; Pocsfalvi, Gabriella. / Coupling immunomagnetic separation on magnetic beads with matrix-assisted laser desorption ionization-time of flight mass spectrometry for detection of staphylococcal enterotoxin B. In: Applied and Environmental Microbiology. 2007 ; Vol. 73, No. 21. pp. 6945-6952.
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