Cotransfer of parthenogenetic embryos improves the pregnancy and implantation of nuclear transfer embryos in mouse

Qinggang Meng, Minkang Wang, Claudia Ana Stanca, Szilard Bodo, A. Dinnyés

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The majority of somatic cell nuclear transfer (SCNT) clones dies in the peri- or postimplantation period. Improvement of the full-term healthy pregnancy rates is a key issue for the economical viability and animal welfare profile of SCNT technology. In this study the effects of cotransfer of parthenogenetic or fertilized embryos on the pregnancy and implantation of SCNT mouse embryos have been investigated. SCNT embryos were produced by transferring cumulus cell nuclei into enucleated B6D2F1 mouse oocytes, whereas parthenogenetically activated (PA) and fertilized embryos were derived from ICR mice by artificial activation with strontium and in vivo fertilization, respectively. SCNT embryos were inferior in their developmental capacity to blastocyst compared to either PA or fertilized embryos. SCNT embryos were transferred alone (SCNT), or cotransferred with two to three PA (SCNT + PA) or fertilized (SCNT + Fert) embryos into the oviducts of an ICR recipient. Both pregnancy and implantation rates originating from clones in the SCNT + PA group were significantly higher than those of SCNT group (p <0.05). The weight of placentas of clones derived from SCNT, SCNT + PA, or SCNT + Fert was in all cases significantly higher than that of fertilized controls (p <0.001). Most of the clones derived from SCNT embryos cotransferred with PA or fertilized embryos survived to adulthood and were fertile and healthy according to histopathological observations. Our results demonstrate in mouse that cotransfer of PA embryos improves the pregnancy and implantation of SCNT embryos without compromising the overall health of the resulting clones.

Original languageEnglish
Pages (from-to)429-434
Number of pages6
JournalCloning and Stem Cells
Volume10
Issue number4
DOIs
Publication statusPublished - Dec 1 2008

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Embryo Transfer
Embryonic Structures
Pregnancy
Clone Cells
Pregnancy Rate
Cumulus Cells
Technology Transfer
Inbred ICR Mouse
Animal Welfare
Strontium
Oviducts
Blastocyst
Cell Nucleus
Fertilization
Placenta
Oocytes

ASJC Scopus subject areas

  • Biotechnology
  • Developmental Biology

Cite this

Cotransfer of parthenogenetic embryos improves the pregnancy and implantation of nuclear transfer embryos in mouse. / Meng, Qinggang; Wang, Minkang; Stanca, Claudia Ana; Bodo, Szilard; Dinnyés, A.

In: Cloning and Stem Cells, Vol. 10, No. 4, 01.12.2008, p. 429-434.

Research output: Contribution to journalArticle

Meng, Qinggang ; Wang, Minkang ; Stanca, Claudia Ana ; Bodo, Szilard ; Dinnyés, A. / Cotransfer of parthenogenetic embryos improves the pregnancy and implantation of nuclear transfer embryos in mouse. In: Cloning and Stem Cells. 2008 ; Vol. 10, No. 4. pp. 429-434.
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