CO2 reactivity measured by perfusion MRI during transient focal cerebral ischemia in rats

L. Oláh, C. Franke, W. Schwindt, M. Hoehn, M. Fisher

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Background and Purpose - CO2 response was examined in rats undergoing 60 minutes of middle cerebral artery occlusion (MCAO) and 4.5 hours of reperfusion. Because it is not clear whether the vasoreactivity improves during reperfusion in parallel with tissue recovery, CO2 response was determined spatially resolved, sequentially in the initially ischemic but later recovered areas and in the permanently damaged areas. Methods - Apparent diffusion coefficient (ADC) maps were calculated from diffusion-weighted images, whereas CO2 reactivity maps were determined from the difference in perfusion signal intensity before and after CO2 stimulation. CO2 reactivity (administration of 6% CO2 for 5 minutes) was expressed in % change of perfusion signal intensity/mm Hg of PCO2 increase. ATP levels of tissue were used as a measure of outcome. The recovered and permanently damaged tissues were differentiated by combined use of end-ischemic ADC map and ATP image at the end of the experiment. Results - The preischemic (control) CO2 reactivity of 3.5±0.9%/mm Hg decreased dramatically during MCAO in the ischemic hemisphere. During reperfusion, it remained 80% of control. Although at the end of the experiment the CO2 reactivity was significantly higher in the recovered tissue than in the permanently damaged tissue (1.15±0.44 and 0.13±0.47%/mm Hg, respectively; P2 challenge permits the investigation of the spatially resolved vascular reactivity during a longitudinal study of cerebral ischemia. Our data suggest that severn ischemia is followed by a prolonged disturbance of CO2 reactivity, despite already normalized energy metabolism.

Original languageEnglish
Pages (from-to)2236-2244
Number of pages9
JournalStroke
Volume31
Issue number9
Publication statusPublished - 2000

Fingerprint

Transient Ischemic Attack
Perfusion
Reperfusion
Middle Cerebral Artery Infarction
Adenosine Triphosphate
Brain Ischemia
Energy Metabolism
Blood Vessels
Longitudinal Studies
Ischemia
Outcome Assessment (Health Care)

Keywords

  • Carbon dioxide
  • Cerebral ischemia, focal
  • Cerebral ischemia, transient
  • Magnetic resonance imaging
  • Rats
  • Reperfusion
  • Vasomotor reactivity

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Neuroscience(all)

Cite this

Oláh, L., Franke, C., Schwindt, W., Hoehn, M., & Fisher, M. (2000). CO2 reactivity measured by perfusion MRI during transient focal cerebral ischemia in rats. Stroke, 31(9), 2236-2244.

CO2 reactivity measured by perfusion MRI during transient focal cerebral ischemia in rats. / Oláh, L.; Franke, C.; Schwindt, W.; Hoehn, M.; Fisher, M.

In: Stroke, Vol. 31, No. 9, 2000, p. 2236-2244.

Research output: Contribution to journalArticle

Oláh, L, Franke, C, Schwindt, W, Hoehn, M & Fisher, M 2000, 'CO2 reactivity measured by perfusion MRI during transient focal cerebral ischemia in rats', Stroke, vol. 31, no. 9, pp. 2236-2244.
Oláh L, Franke C, Schwindt W, Hoehn M, Fisher M. CO2 reactivity measured by perfusion MRI during transient focal cerebral ischemia in rats. Stroke. 2000;31(9):2236-2244.
Oláh, L. ; Franke, C. ; Schwindt, W. ; Hoehn, M. ; Fisher, M. / CO2 reactivity measured by perfusion MRI during transient focal cerebral ischemia in rats. In: Stroke. 2000 ; Vol. 31, No. 9. pp. 2236-2244.
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AU - Franke, C.

AU - Schwindt, W.

AU - Hoehn, M.

AU - Fisher, M.

PY - 2000

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N2 - Background and Purpose - CO2 response was examined in rats undergoing 60 minutes of middle cerebral artery occlusion (MCAO) and 4.5 hours of reperfusion. Because it is not clear whether the vasoreactivity improves during reperfusion in parallel with tissue recovery, CO2 response was determined spatially resolved, sequentially in the initially ischemic but later recovered areas and in the permanently damaged areas. Methods - Apparent diffusion coefficient (ADC) maps were calculated from diffusion-weighted images, whereas CO2 reactivity maps were determined from the difference in perfusion signal intensity before and after CO2 stimulation. CO2 reactivity (administration of 6% CO2 for 5 minutes) was expressed in % change of perfusion signal intensity/mm Hg of PCO2 increase. ATP levels of tissue were used as a measure of outcome. The recovered and permanently damaged tissues were differentiated by combined use of end-ischemic ADC map and ATP image at the end of the experiment. Results - The preischemic (control) CO2 reactivity of 3.5±0.9%/mm Hg decreased dramatically during MCAO in the ischemic hemisphere. During reperfusion, it remained 80% of control. Although at the end of the experiment the CO2 reactivity was significantly higher in the recovered tissue than in the permanently damaged tissue (1.15±0.44 and 0.13±0.47%/mm Hg, respectively; P2 challenge permits the investigation of the spatially resolved vascular reactivity during a longitudinal study of cerebral ischemia. Our data suggest that severn ischemia is followed by a prolonged disturbance of CO2 reactivity, despite already normalized energy metabolism.

AB - Background and Purpose - CO2 response was examined in rats undergoing 60 minutes of middle cerebral artery occlusion (MCAO) and 4.5 hours of reperfusion. Because it is not clear whether the vasoreactivity improves during reperfusion in parallel with tissue recovery, CO2 response was determined spatially resolved, sequentially in the initially ischemic but later recovered areas and in the permanently damaged areas. Methods - Apparent diffusion coefficient (ADC) maps were calculated from diffusion-weighted images, whereas CO2 reactivity maps were determined from the difference in perfusion signal intensity before and after CO2 stimulation. CO2 reactivity (administration of 6% CO2 for 5 minutes) was expressed in % change of perfusion signal intensity/mm Hg of PCO2 increase. ATP levels of tissue were used as a measure of outcome. The recovered and permanently damaged tissues were differentiated by combined use of end-ischemic ADC map and ATP image at the end of the experiment. Results - The preischemic (control) CO2 reactivity of 3.5±0.9%/mm Hg decreased dramatically during MCAO in the ischemic hemisphere. During reperfusion, it remained 80% of control. Although at the end of the experiment the CO2 reactivity was significantly higher in the recovered tissue than in the permanently damaged tissue (1.15±0.44 and 0.13±0.47%/mm Hg, respectively; P2 challenge permits the investigation of the spatially resolved vascular reactivity during a longitudinal study of cerebral ischemia. Our data suggest that severn ischemia is followed by a prolonged disturbance of CO2 reactivity, despite already normalized energy metabolism.

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KW - Cerebral ischemia, focal

KW - Cerebral ischemia, transient

KW - Magnetic resonance imaging

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