Corticotropin-releasing hormone stimulates the in situ generation of mast cells from precursors in the human hair follicle mesenchyme

Natsuho Ito, Koji Sugawara, E. Bódó, Masahiro Takigawa, Nina Van Beek, Taisuke Ito, Ralf Paus

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Hair follicles (HFs) maintain a peripheral, functional equivalent of the hypothalamic-pituitary-adrenal (HPA) axis, whose most proximal element is corticotropin-releasing hormone (CRH). The mast cell (MC)-rich connective-tissue sheath (CTS) of mouse vibrissa HFs harbors MC precursors. Differentiation of these MC precursors into mature MCs can be induced by stem cell factor (SCF). We have investigated whether the MC progenitors of normal human scalp HF CTS respond to stimulation with CRH. Microdissected anagen HFs and full-thickness scalp skin were treated with CRH (10-7 M). CRH treatment induced the degranulation of CTS MCs, in addition to increasing the number of CTS MCs in full-thickness skin and HF organ cultures in situ. In the latter, cells with characteristic MC features emigrated from the CTS. CRH-receptor protein expression in the CTS was colocalized with Kit expression on some CTS MCs in situ. CRH treatment upregulated SCF mRNA and protein expression within the HF epithelium. In skin organ culture, CRH-induced degranulation of CTS MCs was abolished by anti-SCF antibody. We demonstrate that human skin is an extramedullary reservoir for MC precursors, and we have identified a regulatory loop between CRH and SCF signaling. This highlights a previously unpublished finding about neuroendocrine control of human MC biology.

Original languageEnglish
Pages (from-to)995-1004
Number of pages10
JournalJournal of Investigative Dermatology
Volume130
Issue number4
DOIs
Publication statusPublished - Apr 2010

Fingerprint

Hair Follicle
Corticotropin-Releasing Hormone
Mesoderm
Mast Cells
Connective Tissue
Tissue
Stem Cell Factor
Skin
Organ Culture Techniques
Scalp
Cytology
Corticotropin-Releasing Hormone Receptors
Vibrissae
Ports and harbors
Cell Biology
Proteins
Epithelium
Messenger RNA
Antibodies
Therapeutics

ASJC Scopus subject areas

  • Dermatology
  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Corticotropin-releasing hormone stimulates the in situ generation of mast cells from precursors in the human hair follicle mesenchyme. / Ito, Natsuho; Sugawara, Koji; Bódó, E.; Takigawa, Masahiro; Van Beek, Nina; Ito, Taisuke; Paus, Ralf.

In: Journal of Investigative Dermatology, Vol. 130, No. 4, 04.2010, p. 995-1004.

Research output: Contribution to journalArticle

Ito, Natsuho ; Sugawara, Koji ; Bódó, E. ; Takigawa, Masahiro ; Van Beek, Nina ; Ito, Taisuke ; Paus, Ralf. / Corticotropin-releasing hormone stimulates the in situ generation of mast cells from precursors in the human hair follicle mesenchyme. In: Journal of Investigative Dermatology. 2010 ; Vol. 130, No. 4. pp. 995-1004.
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