Correlation of transcriptome profile with electrical activity in temporal lobe epilepsy

Dominique Arion, Michael Sabatini, Travis Unger, Jesús Pastor, Lidia Alonso-Nanclares, Inmaculada Ballesteros-Yáñez, Rafael García Sola, Alberto Muñoz, K. Mirnics, Javier DeFelipe

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

The biology underlying epileptic brain activity in humans is not well understood and likely depends on changes in gene expression. We performed a microarray transcriptome profiling of 12 anterolateral temporal cortical samples originating from five individuals who suffered with temporal lobe epilepsy for at least 10 years. Prior to partial lobectomy, intraoperative electrocorticography was performed on the cortical surface of each patient. These recordings showed characteristic differences in frequency and amplitude that were defined as "spiking" (abnormal) or "non-spiking" (normal). Between the transcriptome of the two sample groups, transferrin (TF) was the most differentially expressed gene. Furthermore, gene expression profiling also revealed a downregulation of multiple GABA system-related genes (GABRA5, GABRB3, ABAT) in the spiking samples and an upregulation of oligodendrocyte and lipid metabolism transcripts (MOG, CA2, CNP, SCD, PLP1, FA2H, ABCA2). In addition, several transcripts related to the classical MAPK cascade showed expression level alterations between the spiking and non-spiking samples (G3BP2, MAPK1, PRKAR1A, and MAP4K4). Out of 12 genes chosen for verification by RT qPCR, 9 showed significant expression changes in the microarray-predicted direction. Furthermore, the microarray and qPCR data were highly correlated (r = 0.98; P 

Original languageEnglish
Pages (from-to)374-387
Number of pages14
JournalNeurobiology of Disease
Volume22
Issue number2
DOIs
Publication statusPublished - May 2006

Fingerprint

Temporal Lobe Epilepsy
Transcriptome
Gene Expression Profiling
Genes
Oligodendroglia
Transferrin
Lipid Metabolism
Human Activities
gamma-Aminobutyric Acid
Up-Regulation
Down-Regulation
Gene Expression
Brain

Keywords

  • DNA microarray
  • Ictal activity
  • qPCR
  • Temporal epilepsy
  • Transcriptome
  • Transferrin

ASJC Scopus subject areas

  • Neurology

Cite this

Arion, D., Sabatini, M., Unger, T., Pastor, J., Alonso-Nanclares, L., Ballesteros-Yáñez, I., ... DeFelipe, J. (2006). Correlation of transcriptome profile with electrical activity in temporal lobe epilepsy. Neurobiology of Disease, 22(2), 374-387. https://doi.org/10.1016/j.nbd.2005.12.012

Correlation of transcriptome profile with electrical activity in temporal lobe epilepsy. / Arion, Dominique; Sabatini, Michael; Unger, Travis; Pastor, Jesús; Alonso-Nanclares, Lidia; Ballesteros-Yáñez, Inmaculada; García Sola, Rafael; Muñoz, Alberto; Mirnics, K.; DeFelipe, Javier.

In: Neurobiology of Disease, Vol. 22, No. 2, 05.2006, p. 374-387.

Research output: Contribution to journalArticle

Arion, D, Sabatini, M, Unger, T, Pastor, J, Alonso-Nanclares, L, Ballesteros-Yáñez, I, García Sola, R, Muñoz, A, Mirnics, K & DeFelipe, J 2006, 'Correlation of transcriptome profile with electrical activity in temporal lobe epilepsy', Neurobiology of Disease, vol. 22, no. 2, pp. 374-387. https://doi.org/10.1016/j.nbd.2005.12.012
Arion D, Sabatini M, Unger T, Pastor J, Alonso-Nanclares L, Ballesteros-Yáñez I et al. Correlation of transcriptome profile with electrical activity in temporal lobe epilepsy. Neurobiology of Disease. 2006 May;22(2):374-387. https://doi.org/10.1016/j.nbd.2005.12.012
Arion, Dominique ; Sabatini, Michael ; Unger, Travis ; Pastor, Jesús ; Alonso-Nanclares, Lidia ; Ballesteros-Yáñez, Inmaculada ; García Sola, Rafael ; Muñoz, Alberto ; Mirnics, K. ; DeFelipe, Javier. / Correlation of transcriptome profile with electrical activity in temporal lobe epilepsy. In: Neurobiology of Disease. 2006 ; Vol. 22, No. 2. pp. 374-387.
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