Correlation of posaconazole minimum fungicidal concentration and time-kill test against nine Candida species

G. Sóczó, G. Kardos, P. M. McNicholas, E. Balogh, L. Gergely, I. Varga, B. Kelentey, L. Majoros

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Objectives: We evaluated the in vitro activity of posaconazole against nine Candida species using minimum fungicidal concentration (MFC) measurements and time-kill methods. Methods: MFCs of posaconazole were determined for 209 clinical isolates (32 Candida albicans, 30 Candida glabrata, 21 Candida tropicalis, 29 Candida krusei, 28 Candida parapsilosis sensu stricto, 50 Candida inconspicua, 13 Candida kefyr, 3 Candida lusitaniae and 3 Candida guilliermondii) and 7 ATCC Candida strains. The following strains were tested in time-kill studies: 3 strains each of C. glabrata, C. kefyr, C. guilliermondii and C. lusitaniae; 2 C. tropicalis; 4 C. albicans; 4 C. inconspicua; 9 C. krusei; 12 C. parapsilosis; and 7 ATCC strains. Results: Posaconazole was fungicidal in both MFC and time-kill experiments (at 2 mg/L within 48 h in time-kill assays) against each C. krusei, C. inconspicua and C. lusitaniae strain and was fungistatic against each C. albicans, C. glabrata, C. tropicalis and C. guilliermondii strain. For the C. parapsilosis strains, posaconazole MFCs were ≤2 mg/L for 20 of the 28 isolates tested; however, in the time-kill tests, even at drug concentrations of 32-64 × MIC, posaconazole was fungistatic against all C. parapsilosis strains tested. Conclusions: MFC values were good predictors of the fungistatic or fungicidal activity of posaconazole, as determined by time-kill curves, for all tested species except C. parapsilosis. Our results highlight the fungicidal action of posaconazole against a number of clinically relevant Candida species.

Original languageEnglish
Pages (from-to)1004-1009
Number of pages6
JournalJournal of Antimicrobial Chemotherapy
Volume60
Issue number5
DOIs
Publication statusPublished - Nov 2007

Fingerprint

Candida
Candida tropicalis
Candida glabrata
Candida albicans
posaconazole
Pharmaceutical Preparations

Keywords

  • Antifungals
  • MFCs
  • Triazoles

ASJC Scopus subject areas

  • Pharmacology
  • Microbiology

Cite this

Correlation of posaconazole minimum fungicidal concentration and time-kill test against nine Candida species. / Sóczó, G.; Kardos, G.; McNicholas, P. M.; Balogh, E.; Gergely, L.; Varga, I.; Kelentey, B.; Majoros, L.

In: Journal of Antimicrobial Chemotherapy, Vol. 60, No. 5, 11.2007, p. 1004-1009.

Research output: Contribution to journalArticle

Sóczó, G. ; Kardos, G. ; McNicholas, P. M. ; Balogh, E. ; Gergely, L. ; Varga, I. ; Kelentey, B. ; Majoros, L. / Correlation of posaconazole minimum fungicidal concentration and time-kill test against nine Candida species. In: Journal of Antimicrobial Chemotherapy. 2007 ; Vol. 60, No. 5. pp. 1004-1009.
@article{c5cba64d89884dabba531ea61f8673e8,
title = "Correlation of posaconazole minimum fungicidal concentration and time-kill test against nine Candida species",
abstract = "Objectives: We evaluated the in vitro activity of posaconazole against nine Candida species using minimum fungicidal concentration (MFC) measurements and time-kill methods. Methods: MFCs of posaconazole were determined for 209 clinical isolates (32 Candida albicans, 30 Candida glabrata, 21 Candida tropicalis, 29 Candida krusei, 28 Candida parapsilosis sensu stricto, 50 Candida inconspicua, 13 Candida kefyr, 3 Candida lusitaniae and 3 Candida guilliermondii) and 7 ATCC Candida strains. The following strains were tested in time-kill studies: 3 strains each of C. glabrata, C. kefyr, C. guilliermondii and C. lusitaniae; 2 C. tropicalis; 4 C. albicans; 4 C. inconspicua; 9 C. krusei; 12 C. parapsilosis; and 7 ATCC strains. Results: Posaconazole was fungicidal in both MFC and time-kill experiments (at 2 mg/L within 48 h in time-kill assays) against each C. krusei, C. inconspicua and C. lusitaniae strain and was fungistatic against each C. albicans, C. glabrata, C. tropicalis and C. guilliermondii strain. For the C. parapsilosis strains, posaconazole MFCs were ≤2 mg/L for 20 of the 28 isolates tested; however, in the time-kill tests, even at drug concentrations of 32-64 × MIC, posaconazole was fungistatic against all C. parapsilosis strains tested. Conclusions: MFC values were good predictors of the fungistatic or fungicidal activity of posaconazole, as determined by time-kill curves, for all tested species except C. parapsilosis. Our results highlight the fungicidal action of posaconazole against a number of clinically relevant Candida species.",
keywords = "Antifungals, MFCs, Triazoles",
author = "G. S{\'o}cz{\'o} and G. Kardos and McNicholas, {P. M.} and E. Balogh and L. Gergely and I. Varga and B. Kelentey and L. Majoros",
year = "2007",
month = "11",
doi = "10.1093/jac/dkm350",
language = "English",
volume = "60",
pages = "1004--1009",
journal = "Journal of Antimicrobial Chemotherapy",
issn = "0305-7453",
publisher = "Oxford University Press",
number = "5",

}

TY - JOUR

T1 - Correlation of posaconazole minimum fungicidal concentration and time-kill test against nine Candida species

AU - Sóczó, G.

AU - Kardos, G.

AU - McNicholas, P. M.

AU - Balogh, E.

AU - Gergely, L.

AU - Varga, I.

AU - Kelentey, B.

AU - Majoros, L.

PY - 2007/11

Y1 - 2007/11

N2 - Objectives: We evaluated the in vitro activity of posaconazole against nine Candida species using minimum fungicidal concentration (MFC) measurements and time-kill methods. Methods: MFCs of posaconazole were determined for 209 clinical isolates (32 Candida albicans, 30 Candida glabrata, 21 Candida tropicalis, 29 Candida krusei, 28 Candida parapsilosis sensu stricto, 50 Candida inconspicua, 13 Candida kefyr, 3 Candida lusitaniae and 3 Candida guilliermondii) and 7 ATCC Candida strains. The following strains were tested in time-kill studies: 3 strains each of C. glabrata, C. kefyr, C. guilliermondii and C. lusitaniae; 2 C. tropicalis; 4 C. albicans; 4 C. inconspicua; 9 C. krusei; 12 C. parapsilosis; and 7 ATCC strains. Results: Posaconazole was fungicidal in both MFC and time-kill experiments (at 2 mg/L within 48 h in time-kill assays) against each C. krusei, C. inconspicua and C. lusitaniae strain and was fungistatic against each C. albicans, C. glabrata, C. tropicalis and C. guilliermondii strain. For the C. parapsilosis strains, posaconazole MFCs were ≤2 mg/L for 20 of the 28 isolates tested; however, in the time-kill tests, even at drug concentrations of 32-64 × MIC, posaconazole was fungistatic against all C. parapsilosis strains tested. Conclusions: MFC values were good predictors of the fungistatic or fungicidal activity of posaconazole, as determined by time-kill curves, for all tested species except C. parapsilosis. Our results highlight the fungicidal action of posaconazole against a number of clinically relevant Candida species.

AB - Objectives: We evaluated the in vitro activity of posaconazole against nine Candida species using minimum fungicidal concentration (MFC) measurements and time-kill methods. Methods: MFCs of posaconazole were determined for 209 clinical isolates (32 Candida albicans, 30 Candida glabrata, 21 Candida tropicalis, 29 Candida krusei, 28 Candida parapsilosis sensu stricto, 50 Candida inconspicua, 13 Candida kefyr, 3 Candida lusitaniae and 3 Candida guilliermondii) and 7 ATCC Candida strains. The following strains were tested in time-kill studies: 3 strains each of C. glabrata, C. kefyr, C. guilliermondii and C. lusitaniae; 2 C. tropicalis; 4 C. albicans; 4 C. inconspicua; 9 C. krusei; 12 C. parapsilosis; and 7 ATCC strains. Results: Posaconazole was fungicidal in both MFC and time-kill experiments (at 2 mg/L within 48 h in time-kill assays) against each C. krusei, C. inconspicua and C. lusitaniae strain and was fungistatic against each C. albicans, C. glabrata, C. tropicalis and C. guilliermondii strain. For the C. parapsilosis strains, posaconazole MFCs were ≤2 mg/L for 20 of the 28 isolates tested; however, in the time-kill tests, even at drug concentrations of 32-64 × MIC, posaconazole was fungistatic against all C. parapsilosis strains tested. Conclusions: MFC values were good predictors of the fungistatic or fungicidal activity of posaconazole, as determined by time-kill curves, for all tested species except C. parapsilosis. Our results highlight the fungicidal action of posaconazole against a number of clinically relevant Candida species.

KW - Antifungals

KW - MFCs

KW - Triazoles

UR - http://www.scopus.com/inward/record.url?scp=35448944908&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=35448944908&partnerID=8YFLogxK

U2 - 10.1093/jac/dkm350

DO - 10.1093/jac/dkm350

M3 - Article

C2 - 17875607

AN - SCOPUS:35448944908

VL - 60

SP - 1004

EP - 1009

JO - Journal of Antimicrobial Chemotherapy

JF - Journal of Antimicrobial Chemotherapy

SN - 0305-7453

IS - 5

ER -