Correlation between the activities of lipoprotein lipase and paraoxonase in type 2 diabetes mellitus

T. Kalmár, I. Seres, Z. Balogh, M. Káplár, G. Winkler, G. Paragh

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

In type 2 diabetes mellitus the decreased catabolism of triglyceride-rich lipoproteins as a consequence of mainly the decreased lipoprotein lipase activity results in hypertriglyceridaemia and other lipoprotein alterations promoting atherosclerosis. The high-density lipoprotein-associated enzyme, paraoxonase, prevents the oxidation of low-density lipoprotein, which is an antiatherogenic effect. Aim: to examine the relation between the activities of enzymes influencing HDL remodelling- LPL and PON- in type 2 diabetes mellitus. Methods: 56 newly diagnosed type 2 diabetic patients and 39 healthy controls were involved in the study. The serum PON activity was measured spectrophotometrically using paraoxone as substrate. PON phenotype was determined by the dual substrate method, PON mass was measured by ELISA. The determination of lipoprotein lipase activity was performed using 3H-triolein. Results: We noticed smaller PON activity decrease in our newly diagnosed diabetic subjects compared to the previous studies which investigated the alteration of enzyme activity after a longer duration of diabetes mellitus. The lipoprotein lipase activity showed a positive correlation with PON activity (r = 0.43; P <0.02). Interestingly, the PON activity of the homozygous-low activity group did not correlate with the LPL activity, while in the heterozygous and homozygous-high activity groups there was a significantly positive correlation (r = 0.51 ; P <0.05) between PON and LPL activity. Conclusion: Besides lipid alterations, the metabolic changes of type 2 diabetes mellitus influence the reduction of the antioxidant capacity of HDL by remodelling HDL and decreasing PON activity via modification of lipoprotein lipase activity, which might contribute to accelerated atherosclerosis.

Original languageEnglish
Pages (from-to)574-580
Number of pages7
JournalDiabetes and Metabolism
Volume31
Issue number6
DOIs
Publication statusPublished - Dec 2005

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Aryldialkylphosphatase
Lipoprotein Lipase
Type 2 Diabetes Mellitus
Lipoproteins
Atherosclerosis
Enzymes
Triolein
Hypertriglyceridemia
HDL Lipoproteins
LDL Lipoproteins
Diabetes Mellitus
Triglycerides
Antioxidants
Enzyme-Linked Immunosorbent Assay
Phenotype
Lipids
Serum

Keywords

  • Lipoprotein lipase
  • Paraoxonase
  • Triglycerides
  • Type 2 diabetes mellitus

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Correlation between the activities of lipoprotein lipase and paraoxonase in type 2 diabetes mellitus. / Kalmár, T.; Seres, I.; Balogh, Z.; Káplár, M.; Winkler, G.; Paragh, G.

In: Diabetes and Metabolism, Vol. 31, No. 6, 12.2005, p. 574-580.

Research output: Contribution to journalArticle

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