Correlation between detergent activity and anti-herpes simplex virus-2 activity of commercially available vaginal gels

Andrea Szöllosi, Tímea Raffai, Anita Bogdanov, Valéria Endrész, László Párducz, Ferenc Somogyvári, László Janovák, Katalin Burián, Dezso P. Virok

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Abstract

Objective: Herpes simplex virus-2 (HSV-2) infections are almost exclusively sexually transmitted. The presence of vaginal gels during sexual activity may have a significant positive or negative impact on viral transmission. Therefore we investigated three off-the-shelf vaginal lubricants and one pH restoring gel to evaluate their impact on HSV-2 replication. Results: HeLa cells were infected with untreated virions and virions incubated with the particular gels. The accumulation of viral genomes was monitored by quantitative PCR (qPCR) method at 24 h post infection. Two of the tested gels had no significant effect on HSV-2 replication at the maximum applied concentration, while two had a strong inhibitory effect (~ 98% reduction of replication). The replication inhibitory effect was observed at various multiplicity of infection (MOI 0.4-6.4) and the two inhibitory gels were also capable of inhibiting the HSV-2 induced cytopathic effect on HeLa cells. The surface tension decreasing activity- A n indication of detergent activity-was strongly correlated with the anti-HSV-2 activity of the gels (R2: 0.88). Our results indicate that off-the-shelf vaginal gels have a markedly different anti-HSV-2 activity that may influence HSV-2 transmission.

Original languageEnglish
Article number52
JournalBMC Research Notes
Volume13
Issue number1
DOIs
Publication statusPublished - Jan 31 2020

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Keywords

  • Gel
  • Herpes
  • HSV
  • qPCR
  • Replication
  • Simplex
  • STD
  • Transmission
  • Vaginal

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Szöllosi, A., Raffai, T., Bogdanov, A., Endrész, V., Párducz, L., Somogyvári, F., Janovák, L., Burián, K., & Virok, D. P. (2020). Correlation between detergent activity and anti-herpes simplex virus-2 activity of commercially available vaginal gels. BMC Research Notes, 13(1), [52]. https://doi.org/10.1186/s13104-020-4918-4