Corneal ulcer/keratitis derived Aspergillus flavus & Aspergillus tamarii and their RAPD-PCR typing

Bader Alshehri, Palanisamy Manikandan, Ahmed Abdel-hadi, Abdul Aziz Bin Dukhyil, Mohammed Alaidarous, Saeed Banawas, Raed Abdullah Alharbi, Saleh Abdullah Aloyuni, Csaba Vágvölgyi, László Kredics, Sándor Kocsubé, Esam S. Al-Malki, Rajendran Vijayakumar, Kanesan Panneer Selvam, Coimbatore Subramanian Shobana

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Abstract

The aim of the study was investigate the DNA polymorphisms of Aspergillus flavus and Aspergillus tamarii isolated from regional fungal keratitis cases based on random amplified polymorphic DNA (RAPD) analyses. Thirty-two A. flavus and six A. tamarii isolates were identified using conventional macroscopic and microscopic features. Five decamer primers were used to amplify the genomic DNA sequences of the test isolates, and 140 total RAPD patterns were generated among them. From each of the 38 isolates, a range of 15–30 RAPD types were revealed. All of these were isolated from male farmers from Tamil Nadu. In the future, RAPD analyses could be employed as a reliable and rapid technique to differentiate among keratitis isolates of fungal pathogens.

Original languageEnglish
JournalJournal of King Saud University - Science
DOIs
Publication statusAccepted/In press - Jan 1 2020

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Keywords

  • Aspergillus flavus
  • Corneal ulcer
  • Eye infection
  • Keratitis
  • Molecular typing
  • RAPD PCR

ASJC Scopus subject areas

  • General

Cite this

Alshehri, B., Manikandan, P., Abdel-hadi, A., Dukhyil, A. A. B., Alaidarous, M., Banawas, S., Alharbi, R. A., Aloyuni, S. A., Vágvölgyi, C., Kredics, L., Kocsubé, S., Al-Malki, E. S., Vijayakumar, R., Selvam, K. P., & Shobana, C. S. (Accepted/In press). Corneal ulcer/keratitis derived Aspergillus flavus & Aspergillus tamarii and their RAPD-PCR typing. Journal of King Saud University - Science. https://doi.org/10.1016/j.jksus.2020.02.019