Copper(II) interaction with the Human Prion 103–112 fragment – Coordination and oxidation

Gizella Csire, Lajos Nagy, K. Várnagy, C. Kállay

Research output: Contribution to journalArticle

8 Citations (Scopus)


The prion protein (PrP) is a membrane-anchored cell surface glycoprotein containing 231 amino acids. It has been associated with a group of neurodegenerative disorders. Copper(II) interaction with the Human Prion 103–112 fragment and its mutants has been studied with various techniques. The studied human prion fragment contains both histidine and methionine residues, while methionine residues are systematically replaced or displaced in the studied mutants. pH-potentiometric, UV–Vis and circular dicroism spectroscopic techniques were applied to study the stoichiometry, stability and structure of the copper(II) complexes, while HPLC-MS and MS/MS were used for identifying the products of copper(II) catalyzed oxidation. The complex formation reactions of the studied ligands are rather similar; only 1:1 complexes are formed, where the imidazole nitrogen of the histidine residue is the main binding site beside the amide nitrogens of the peptide chain. The only difference is, that in the peptides which contain methionine in position 109, in addition to the (Nim,N,N) coordination mode, a weak interaction of thioether sulfur atoms can be supposed. The mutant peptide which does not contain methionine did not undergo oxidation, only the fragmentation of the peptide chain was perceived. However, in the case of methionine containing peptides, the peptide chain was not cleaved; but the oxidation of methionine to methionine sulfoxide occurred.

Original languageEnglish
Pages (from-to)195-201
Number of pages7
JournalJournal of Inorganic Biochemistry
Publication statusPublished - May 1 2017

ASJC Scopus subject areas

  • Biochemistry
  • Inorganic Chemistry

Fingerprint Dive into the research topics of 'Copper(II) interaction with the Human Prion 103–112 fragment – Coordination and oxidation'. Together they form a unique fingerprint.

  • Cite this