COP1 contributes to UVB-induced signaling in human keratinocytes

Ágnes Kinyó, Zsuzsanna Kiss-László, Szabolcs Hambalkó, Attila Bebes, M. Kiss, M. Széll, Z. Bata-Csörgő, Ferenc Nagy, L. Kemény

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

UVB irradiation has been shown to trigger a broad range of changes in gene expression in human skin; however, factors governing these events are still not well understood. In this study, we show that human constitutive photomorphogenic protein-1 (huCOP1), an E3 ligase, contributes to the orchestration of UVB response of keratinocytes. Accordingly, our data show that (i) huCOP1 protein is expressed both in the nucleus and in the cytoplasm of cultured keratinocytes, (ii) UVB reduces the levels of the huCOP1 mRNA and protein, and (iii) induces changes in the subcellular localization of huCOP1. Finally, we show that gene-specific silencing of huCOP1 induces the accumulation of the tumor suppressor p53 protein, which is further increased after UVB irradiation.

Original languageEnglish
Pages (from-to)541-545
Number of pages5
JournalJournal of Investigative Dermatology
Volume130
Issue number2
DOIs
Publication statusPublished - Feb 2010

Fingerprint

Keratinocytes
Irradiation
Tumor Suppressor Protein p53
Ubiquitin-Protein Ligases
Gene Silencing
Gene expression
Skin
Cytoplasm
Proteins
Genes
human RFWD2 protein
Gene Expression
Messenger RNA

ASJC Scopus subject areas

  • Dermatology
  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

COP1 contributes to UVB-induced signaling in human keratinocytes. / Kinyó, Ágnes; Kiss-László, Zsuzsanna; Hambalkó, Szabolcs; Bebes, Attila; Kiss, M.; Széll, M.; Bata-Csörgő, Z.; Nagy, Ferenc; Kemény, L.

In: Journal of Investigative Dermatology, Vol. 130, No. 2, 02.2010, p. 541-545.

Research output: Contribution to journalArticle

Kinyó, Ágnes ; Kiss-László, Zsuzsanna ; Hambalkó, Szabolcs ; Bebes, Attila ; Kiss, M. ; Széll, M. ; Bata-Csörgő, Z. ; Nagy, Ferenc ; Kemény, L. / COP1 contributes to UVB-induced signaling in human keratinocytes. In: Journal of Investigative Dermatology. 2010 ; Vol. 130, No. 2. pp. 541-545.
@article{85d1d1f551ba4899bf9813a80fbe2ed1,
title = "COP1 contributes to UVB-induced signaling in human keratinocytes",
abstract = "UVB irradiation has been shown to trigger a broad range of changes in gene expression in human skin; however, factors governing these events are still not well understood. In this study, we show that human constitutive photomorphogenic protein-1 (huCOP1), an E3 ligase, contributes to the orchestration of UVB response of keratinocytes. Accordingly, our data show that (i) huCOP1 protein is expressed both in the nucleus and in the cytoplasm of cultured keratinocytes, (ii) UVB reduces the levels of the huCOP1 mRNA and protein, and (iii) induces changes in the subcellular localization of huCOP1. Finally, we show that gene-specific silencing of huCOP1 induces the accumulation of the tumor suppressor p53 protein, which is further increased after UVB irradiation.",
author = "{\'A}gnes Kiny{\'o} and Zsuzsanna Kiss-L{\'a}szl{\'o} and Szabolcs Hambalk{\'o} and Attila Bebes and M. Kiss and M. Sz{\'e}ll and Z. Bata-Cs{\"o}rgő and Ferenc Nagy and L. Kem{\'e}ny",
year = "2010",
month = "2",
doi = "10.1038/jid.2009.286",
language = "English",
volume = "130",
pages = "541--545",
journal = "Journal of Investigative Dermatology",
issn = "0022-202X",
publisher = "Nature Publishing Group",
number = "2",

}

TY - JOUR

T1 - COP1 contributes to UVB-induced signaling in human keratinocytes

AU - Kinyó, Ágnes

AU - Kiss-László, Zsuzsanna

AU - Hambalkó, Szabolcs

AU - Bebes, Attila

AU - Kiss, M.

AU - Széll, M.

AU - Bata-Csörgő, Z.

AU - Nagy, Ferenc

AU - Kemény, L.

PY - 2010/2

Y1 - 2010/2

N2 - UVB irradiation has been shown to trigger a broad range of changes in gene expression in human skin; however, factors governing these events are still not well understood. In this study, we show that human constitutive photomorphogenic protein-1 (huCOP1), an E3 ligase, contributes to the orchestration of UVB response of keratinocytes. Accordingly, our data show that (i) huCOP1 protein is expressed both in the nucleus and in the cytoplasm of cultured keratinocytes, (ii) UVB reduces the levels of the huCOP1 mRNA and protein, and (iii) induces changes in the subcellular localization of huCOP1. Finally, we show that gene-specific silencing of huCOP1 induces the accumulation of the tumor suppressor p53 protein, which is further increased after UVB irradiation.

AB - UVB irradiation has been shown to trigger a broad range of changes in gene expression in human skin; however, factors governing these events are still not well understood. In this study, we show that human constitutive photomorphogenic protein-1 (huCOP1), an E3 ligase, contributes to the orchestration of UVB response of keratinocytes. Accordingly, our data show that (i) huCOP1 protein is expressed both in the nucleus and in the cytoplasm of cultured keratinocytes, (ii) UVB reduces the levels of the huCOP1 mRNA and protein, and (iii) induces changes in the subcellular localization of huCOP1. Finally, we show that gene-specific silencing of huCOP1 induces the accumulation of the tumor suppressor p53 protein, which is further increased after UVB irradiation.

UR - http://www.scopus.com/inward/record.url?scp=75549086193&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=75549086193&partnerID=8YFLogxK

U2 - 10.1038/jid.2009.286

DO - 10.1038/jid.2009.286

M3 - Article

C2 - 19741714

AN - SCOPUS:75549086193

VL - 130

SP - 541

EP - 545

JO - Journal of Investigative Dermatology

JF - Journal of Investigative Dermatology

SN - 0022-202X

IS - 2

ER -