Coordination-chemical study of polypeptides-II. Equilibrium study of the zinc complex formation of synthetic αH-corticotropin1-32

K. Burger, F. Gaizer, I. Zay, M. Pékli, B. Noszál

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Separation, with the aid of a dialysis membrane, of the measuring electrode from the solution to be studied, enabled a zinc amalgam electrode system to be developed which is suitable for determination of zinc ion activity, even in the presence of macromolecules which poison the electrode. Equilibrium measurements with this apparatus and computer evaluation of the experimental results allowed determination of the compositions of the complexes formed between the zinc ion and ACTH1-32, a 32 aminoacid-containing fragment of corticotropin (ACTH), in aqueous solution, and also their conditional stability constants in a system of pH 5.9. On the basis of earlier independent deprotonation and polarographic equilibrium investigations, proposals have been put forward as to which of the large number of donor groups of the polypeptide take part in the formation of the individual complexes.

Original languageEnglish
Pages (from-to)725-728
Number of pages4
JournalJournal of Inorganic and Nuclear Chemistry
Volume40
Issue number4
DOIs
Publication statusPublished - 1978

Fingerprint

Polypeptides
polypeptides
Zinc
zinc
Adrenocorticotropic Hormone
Peptides
Electrodes
Dialysis membranes
Ions
electrodes
Mercury amalgams
Deprotonation
adrenocorticotropin (ACTH)
Poisons
Macromolecules
mercury amalgams
poisons
dialysis
macromolecules
proposals

Cite this

Coordination-chemical study of polypeptides-II. Equilibrium study of the zinc complex formation of synthetic αH-corticotropin1-32. / Burger, K.; Gaizer, F.; Zay, I.; Pékli, M.; Noszál, B.

In: Journal of Inorganic and Nuclear Chemistry, Vol. 40, No. 4, 1978, p. 725-728.

Research output: Contribution to journalArticle

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AU - Noszál, B.

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