11β-Hydroxysteroid dehydrogenase type 1 (11βHSD1) is a NADP(H)-dependent oxidoreductase of the ER lumen, which may have an important role in the pathogenesis of metabolic syndrome. Here, the functional coupling of 11β-hydroxysteroid dehydrogenase type 1 and hexose-6-phosphate dehydrogenase (H6PDH) was investigated in rat liver microsomal vesicles. The results demonstrate the existence of a separate intraluminal pyridine nucleotide pool in the hepatic endoplasmic reticulum and a close cooperation between 11βHSD1 and H6PDH based on their co-localization and the mutual generation of cofactors for each other.
- 11β-Hydroxysteroid dehydrogenase type 1
- Hexose-6-phosphate dehydrogenase
- Pyridine nucleotides
- Rat liver microsomes
ASJC Scopus subject areas
- Molecular Biology