Cooperative roles of vertebrate Fbh1 and Blm DNA helicases in avoidance of crossovers during recombination initiated by replication fork collapse

Masaoki Kohzaki, Atsushi Hatanaka, Eiichiro Sonoda, Mitsuyoshi Yamazoe, Koji Kikuchi, Vu Trung Nguyen, D. Szüts, Julian E. Sale, Hideo Shinagawa, Masami Watanabe, Shunichi Takeda

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Fbh1 (F-box DNA helicase 1) orthologues are conserved from Schizosaccharomyces pombe to chickens and humans. Here, we report the disruption of the FBH1 gene in DT40 cells. Although the yeast fbh1 mutant shows an increase in sensitivity to DNA damaging agents, FBH1-/- DT40 clones show no prominent sensitivity, suggesting that the loss of FBH1 might be compensated by other genes. However, FBH1-/- cells exhibit increases in both sister chromatid exchange and the formation of radial structures between homologous chromosomes without showing a defect in homologous recombination. This phenotype is reminiscent of BLM-/- cells and suggests that Fbh1 may be involved in preventing extensive strand exchange during homologous recombination. In addition, disruption of RAD54, a major homologous recombination factor in FBH1-/- cells, results in a marked increase in chromosome-type breaks (breaks on both sister chromatids at the same place) following replication fork arrest. Further, FBH1BLM cells showed additive increases in both sister chromatid exchange and the formation of radial chromosomes. These data suggest that Fbh1 acts in parallel with Bloom helicase to control recombination-mediated double-strand-break repair at replication blocks and to reduce the frequency of crossover.

Original languageEnglish
Pages (from-to)2812-2820
Number of pages9
JournalMolecular and Cellular Biology
Volume27
Issue number8
DOIs
Publication statusPublished - Apr 2007

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DNA Helicases
Genetic Recombination
Vertebrates
Homologous Recombination
Sister Chromatid Exchange
Chromosomes
Chromosome Breakage
Chromatids
Schizosaccharomyces
Genes
Chickens
Clone Cells
Yeasts
Phenotype
DNA

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Cooperative roles of vertebrate Fbh1 and Blm DNA helicases in avoidance of crossovers during recombination initiated by replication fork collapse. / Kohzaki, Masaoki; Hatanaka, Atsushi; Sonoda, Eiichiro; Yamazoe, Mitsuyoshi; Kikuchi, Koji; Nguyen, Vu Trung; Szüts, D.; Sale, Julian E.; Shinagawa, Hideo; Watanabe, Masami; Takeda, Shunichi.

In: Molecular and Cellular Biology, Vol. 27, No. 8, 04.2007, p. 2812-2820.

Research output: Contribution to journalArticle

Kohzaki, M, Hatanaka, A, Sonoda, E, Yamazoe, M, Kikuchi, K, Nguyen, VT, Szüts, D, Sale, JE, Shinagawa, H, Watanabe, M & Takeda, S 2007, 'Cooperative roles of vertebrate Fbh1 and Blm DNA helicases in avoidance of crossovers during recombination initiated by replication fork collapse', Molecular and Cellular Biology, vol. 27, no. 8, pp. 2812-2820. https://doi.org/10.1128/MCB.02043-06
Kohzaki, Masaoki ; Hatanaka, Atsushi ; Sonoda, Eiichiro ; Yamazoe, Mitsuyoshi ; Kikuchi, Koji ; Nguyen, Vu Trung ; Szüts, D. ; Sale, Julian E. ; Shinagawa, Hideo ; Watanabe, Masami ; Takeda, Shunichi. / Cooperative roles of vertebrate Fbh1 and Blm DNA helicases in avoidance of crossovers during recombination initiated by replication fork collapse. In: Molecular and Cellular Biology. 2007 ; Vol. 27, No. 8. pp. 2812-2820.
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