Convergent evolution and orphan genes in the Fur4p-like family and characterization of a general nucleoside transporter in Aspergillus nidulans

Z. Hamari, Sotiris Amillis, Christine Drevet, Angeliki Apostolaki, C. Vágvölgyi, George Diallinas, Claudio Scazzocchio

Research output: Contribution to journalArticle

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Abstract

The function of seven paralogues phylogenetically related to the Saccharomyces cerevisiae Fur4p together with a number of functionally related transporters present in Aspergillus nidulans has been investigated. After deletion of the cognate genes we checked the incorporation of radiolabelled substrates, utilization of nitrogen sources, resistance to toxic analogues and supplementation of auxotrophies. FurA and FurD encode allantoin and uracil transporters respectively. No function was found for FurB, FurC, FurE, FurF and FurG. As we failed to identify Fur-related transporters for uridine, pyridoxine or thiamine, we deleted other possible candidates for these functions. A FCY2-like gene carrying in its 5′ UTR a putative thiamine pyrophosphate riboswitch, and which encodes a protein similar to the pyridoxine transporter of yeast (Tpn1p), does not encode either a major thiamine or a pyridoxine transporter. CntA, a member of the concentrative nucleoside transporter family, is a general nucleoside permease, while no function was found for PnpA, a member of the equilibrative transporter family. Phylogenetic analysis shows that within the ascomycetes, the same transport activity could be catalysed by totally unrelated proteins and that within the Fur subfamily convergent evolution towards uracil and allantoin transport activity has occurred at least three and two independent times respectively.

Original languageEnglish
Pages (from-to)43-57
Number of pages15
JournalMolecular Microbiology
Volume73
Issue number1
DOIs
Publication statusPublished - Jul 2009

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Nucleoside Transport Proteins
Aspergillus nidulans
Pyridoxine
Allantoin
Uracil
Thiamine
Riboswitch
Thiamine Pyrophosphate
Genes
Ascomycota
Membrane Transport Proteins
Poisons
5' Untranslated Regions
Uridine
Gene Deletion
Nucleosides
Saccharomyces cerevisiae
Proteins
Nitrogen
Yeasts

ASJC Scopus subject areas

  • Molecular Biology
  • Microbiology

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Convergent evolution and orphan genes in the Fur4p-like family and characterization of a general nucleoside transporter in Aspergillus nidulans. / Hamari, Z.; Amillis, Sotiris; Drevet, Christine; Apostolaki, Angeliki; Vágvölgyi, C.; Diallinas, George; Scazzocchio, Claudio.

In: Molecular Microbiology, Vol. 73, No. 1, 07.2009, p. 43-57.

Research output: Contribution to journalArticle

Hamari, Z. ; Amillis, Sotiris ; Drevet, Christine ; Apostolaki, Angeliki ; Vágvölgyi, C. ; Diallinas, George ; Scazzocchio, Claudio. / Convergent evolution and orphan genes in the Fur4p-like family and characterization of a general nucleoside transporter in Aspergillus nidulans. In: Molecular Microbiology. 2009 ; Vol. 73, No. 1. pp. 43-57.
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